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CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit

Product Information

  • Product name

    CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit

  • Application notes

    CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit provides a simple and easy colorimetric assay for the study of Hydroxyl radical scavenging Capacity in various sample. In this assay, H2O2/ Fe2+ produces hydroxyl radical through Fenton reaction, and oxidizes Fe2+ to Fe3+, resulting in the decrease of 536nm absorbance. The Inhibition rate of samples upon the decrease of 536nm absorbance can reflect the Hydroxyl Free Radical Scavenging Capacity of the sample.

Product Properties

  • Kit components

    • Lysis Buffer
    • Assay Buffer
    •Chromogen A
    • Chromogen B
    • H2O2 (0.03%)

  • Features & Benefits

    • Determination the Hydroxyl Free Radical Scavenging Capacity in various samples, including serum, plasma, tissue lysates and other biological fluids.

  • Usage notes

    • If not assayed immediately, samples can be stored at -80°C.
    • In order to compare the hydroxyl radical scavenging capacity of different samples, serum, tissue homogenate, juice and other liquid samples must add the same volume for the same batch. Exact or Drug must be diluted to the same concentration.

  • Storage instructions

    Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The hydroxyl radical, •OH, is the neutral form of the hydroxide ion (OH−), highly reactive (easily becoming hydroxyl groups) and consequently short-lived. However, they form an important part of radical chemistry. Most notably hydroxyl radicals are produced from the decomposition of hydroperoxides (ROOH) or, in atmospheric chemistry, by the reaction of excited atomic oxygen with water. The hydroxyl radical can damage virtually all types of macromolecules: carbohydrates, nucleic acids (mutations), lipids (lipid peroxidation) and amino acids (e.g. conversion of Phe to m-Tyrosine and o-Tyrosine), resulting in cell structure and function damage, and then lead to metabolic disorders. The scavenging ability of hydroxyl radical represents the antioxidant ability, widely used in the research of antioxidant health products and drugs.

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CheKine™ Hydroxyl Free Radical Scavenging Capacity Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1090

  • Size
  • Price
  • 48T $40
  • 96T $60

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Fax: +86-27-59716788
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