|Product name||CheKine™ Hydrogen Peroxide (H2O2) Assay Kit|
|Applications notes||CheKine™ Hydrogen Peroxide Assay Kit provides a simple and easy colorimetric assay for measuring hydrogen peroxide in serum, plasma, cell culture supernatants, tissue/cell lysates and other biological fluids. In the assay, H2O2 oxidize ferrous to ferric ion, and xylenol orange binds ferric ion with high selectivity to form a colored (purple) product that can be measured by colorimetric method at OD 580 nm. The Hydrogen Peroxide present in the sample is proportional to the signal obtained.|
|Kit components||• Reaction Buffer
• H2O2 Standard (1M)
• Assay Buffer (10X)
|Features & Benefits||• Determination of Hydrogen Peroxide in serum, plasma, cell/tissue samples, urine and other biological fluids.
• Detect biological samples at a level of 1-20 µM.
• Simple procedures, fase and convenient, and more stable.
|Usage notes||• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.
• Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80°C for one month.
|Storage instructions||Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Hydrogen Peroxide (H2O2), a reactive oxygen metabolic by-product, which serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neurodegenerative diseases, Down’s syndrome and immune system diseases.|
Fig. Standard Curve of Hydrogen Peroxide Assay. The y-axis is OD580 and the x-axis is Hydrogen Peroxide concentration (μM).
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