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CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit

Product Information

  • Product name

    CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit

  • Application notes

    GR can catalyze the reduction of NADPH to GSSG to regenerate GSH, and NADPH dehydrogenates to produce NADP +; NADPH has a characteristic absorption peak at 340 nm, while NADP + has no absorption peak at this wavelength; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GR activity.

Fig. CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit

Product Properties

  • Kit components

    • Assay Buffer
    • Substrate
    • GR Cofactor

  • Features & Benefits

    • Determination of GR activity in serum, plasma, tissue/cell lysates and other biological fluids.

  • Usage notes

    • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
    • The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
    • In the detection of GR activity in cells, the cell number must be between 3-5 x 106, and the extraction of GR in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
    • Before measurement, one or two samples should be used as a pre-experiment to ensure that the change in absorbance within 180 s is linear.
    • Generally, mammalian tissue must be diluted 2 to 5 times with Assay Buffer.
    • The measuring process should be operated quickly and only 1 ~ 2 blank well should be needed.
    • The reaction temperature has influence on the result. Keep the temperature at 25°C (for general species) or 37°C (for mammals).

  • Storage instructions

    Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    GR is a flavin protein redox enzyme widely present in eukaryotes and prokaryotes. GR catalyzes the reduction of GSSG to GSH. It is one of the key enzymes in the glutathione redox cycle (generally GR is replaced by TrxR in insects). GR catalyzes the reduction of GSSG by NADPH to GSH, which helps maintain the GSH / GSSG ratio in the body. GR plays a key role in scavenging reactive oxygen species in response to oxidative stress, and GR is also involved in the ascorbate-glutathione cycle pathway.

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CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

Cat #: KTB1620

  • Size
  • Price
  • 48T $60
  • 96T $90
  • 192T $156

Contact information

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  •  Fax: +86-27-59716788
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