CheKine™ Glucose Oxidase Activity (GOD)Assay Kit

Product Information

  • Product name

    CheKine™ Glucose Oxidase Activity (GOD)Assay Kit

  • Application notes

    CheKine™ Glucose Oxidase Activity Assay Kit provides a simple and easy colorimetric assay for detecting Glucose Oxidase Activity in biological samples such as in serum or plasma, cells, urine, other body fluid, food, growth medium. In this assay, GOD activity is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with chromogen in an acid condition, produce a product that can be measured at OD 580 nm. Therefore, the glucose oxidase activity present in the sample is proportional to the signal obtained.

Fig. Standard Curve of GOD assay. The y-axis is OD580 and the x-axis is H2O2 concentration ( μM).

Product Properties

  • Kit components

    • Assay Buffer (20x)
    • Glucose (0.2 M)
    •H2O2 Standard (0.88 M)
    • Chromogen
    • Glucose Oxidase (control)

  • Features & Benefits

    • Optimized procedure for detecting Glucose Oxidase Activity in biological samples such as in serum or plasma, cells, urine, other body fluid, food, growth medium.
    • Simple and easy colorimetric assay.
    • Quick and convenient.

  • Calibration range

    0.05 - 2 U/L

  • Limit of detection

    0.05 U/L

  • Usage notes

    • Briefly centrifuge small vials at low speed prior to opening.
    • Performing several dilutions of your sample to ensure the readings are within the standard value range.
    • Use fresh samples. If not assayed immediately, samples can be stored at -80°C for one month.

  • Storage instructions

    Storage at –20°C and keep from light. Kit has a storage time of 12 months from receipt.

Additional Information

  • Background

    The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. This enzyme is produced by certain species of insects, fungi, and bacteria. Glucose oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose.

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CheKine™ Glucose Oxidase Activity (GOD)Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1310

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  • 48T $40
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  • 480T $180

Contact information

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