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CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit

CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit

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Specification

Product name CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting glucose-6-phosphate dehydrogenase (G6PDH) activity in a variety of biological samples such as serum, plasma, tissues, cells , plants and bacteria. In the assay, G6PDH present in the sample converts NADP+ to NADPH, which has an absorbance at 340 nm. The absorbance of NADPH is proportional to the G6PDH activity present in the sample.

Product Properties

Kit components • Assay Buffer
• 6-Phosphogluconic acid
• NADP+
Features & Benefits • Determination of G6PDH activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage instructions Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction. This enzyme participates in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage from compounds like hydrogen peroxide.

Image & description

Fig. CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit

Fig. CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit

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