|Product name||CheKine™ Gamma-Glutamyl Transpeptidase (GGT) Activity Colorimetric Assay Kit|
|Applications notes||The kit provides a simple method for detecting γ-GT enzyme activity in a variety of biological samples such as tissues, cell,serum, bacterial. In the assay, γ-GT catalyzes the transfer of γ-glutamyl group in glutamyl p-nitroaniline to N-glycylglycine to generate p-nitroaniline, which has characteristic light absorption at 405nm; Calculate γ-GT enzyme activity by measuring the increase rate of light absorption at 405nm.|
|Kit components||• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
|Features & Benefits||• Determination of γ-GT enzyme activity in a variety of biological samples such as tissues, cell,serum, bacterial.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
|Usage notes||• Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
|Storage instructions||Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||γ-GT is a key enzyme in the γ-glutamyl cycle and catalyzes the degradation of GSH. γ-GT catalyzes the transfer of the γ-glutamyl group on GSH or other γ-glutamyl compounds to the receptor, and can also catalyze the hydrolysis of GSH and other γ-glutamyl compounds to produce glutamate, and is outside the cell Glutathione plays an important role in the metabolism.|
Fig. CheKine™ Gamma-Glutamyl Transpeptidase (GGT) Activity Colorimetric Assay Kit.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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