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CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

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Specification

Product name CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit
Applications notes DAO catalyzed resin amine to produce aldehyde and hydrogen peroxide, and add excessive horseradish peroxidase exogenous to catalyze hydrogen peroxide to oxidize o-anisamine to produce colored substances which has a characteristic absorption peak at 460 nm. DAO activity is calculated by measuring the absorbance increase rate of this wavelength. It can test samples of tissue, cells, bacteria, plants and serum (plasma).
Alternative Amine oxidase, copper-containing, 1; AOC1; Histaminase

Product Properties

Kit components • Extraction Buffer
•ReagentⅠ
• ReagentⅡ
• ReagentⅢ
Features & Benefits • Determination of DAO activity in a variety of biological samples such as animal tissues, plant tissues, cell ,serum (plasma), bacterial.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
Usage notes • Do not mix or substitute reagents or materials from other kit lots or different vendors, or it may cause abnormal result.
•Avoid foaming or bubbles when mixing or reconstituting components.
• To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
•Ensure all reagents and solutions are at the appropriate temperature before starting the assay.
Storage instructions Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background DAO(EC1.4.3.6) is widely present in animals (intestinal mucosa, lung, liver, kidney), plants, microorganisms. It catalyzes polyamine and oxidized to aldehydes, Its activity is closely related to nucleic acid and protein synthesis and can reflect the integrity of the intestinal mechanical barrier and the degree of damage.
Alternative Amine oxidase, copper-containing, 1; AOC1; Histaminase

Image & description

Fig. CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

Fig. CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

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