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CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

Product Information

  • Product name

    CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

  • Application notes

    DAO catalyzed resin amine to produce aldehyde and hydrogen peroxide, and add excessive horseradish peroxidase exogenous to catalyze hydrogen peroxide to oxidize o-anisamine to produce colored substances which has a characteristic absorption peak at 460 nm. DAO activity is calculated by measuring the absorbance increase rate of this wavelength. It can test samples of tissue, cells, bacteria, plants and serum (plasma).

Fig. CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit

Product Properties

  • Kit components

    • Extraction Buffer   •ReagentⅠ   • ReagentⅡ   • ReagentⅢ

  • Features & Benefits

    • Determination of DAO activity in a variety of biological samples such as animal tissues, plant tissues, cell ,serum (plasma), bacterial. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

  • Usage notes

    • Do not mix or substitute reagents or materials from other kit lots or different vendors, or it may cause abnormal result.
    •Avoid foaming or bubbles when mixing or reconstituting components.
    • To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
    •Ensure all reagents and solutions are at the appropriate temperature before starting the assay.

  • Storage instructions

    Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    DAO(EC1.4.3.6) is widely present in animals (intestinal mucosa, lung, liver, kidney), plants, microorganisms. It catalyzes polyamine and oxidized to aldehydes, Its activity is closely related to nucleic acid and protein synthesis and can reflect the integrity of the intestinal mechanical barrier and the degree of damage.

  • Alternative names

    Amine oxidase, copper-containing, 1; AOC1; Histaminase

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CheKine™ Diamine Oxidase (DAO) Activity Colorimetric Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1220

  • Size
  • Price
  • 48T $129
  • 96T $229

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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