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CheKine™ Coenzyme Ⅰ NAD(H) Assay Kit

CheKine™ Coenzyme Ⅰ NAD(H) Assay Kit

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Specification

Product name CheKine™ Coenzyme Ⅰ NAD(H) Assay Kit
Applications notes Abbkine CheKine™ Coenzyme Ⅰ NAD(H) Assay Kitprovides a convenient tool for sensitive detection of the NAD and NADH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NADP+/NADPH), in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NAD+ /NADH concentration in the sample.

Product Properties

Kit components • Assay Buffer
• EtOH Solution
• WST-8 Solution
• Enhancer
• NAD Cycling Enzyme Mix
• NAD standard (10 mM)
• NAD Extraction Buffer
• NADH Extraction Buffer
Features & Benefits • Detect NAD+/NADH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.
Calibration range 0.78 µM-50 µM
Usage notes • Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
Storage instructions Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.

Image & description

Fig. Standard Curve of NAD in 96-well plate assay. The y-axis is ΔOD and the x-axis is NAD concentration (uM).

Fig. Standard Curve of NAD in 96-well plate assay. The y-axis is ΔOD and the x-axis is NAD concentration (uM).

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