|Product name||CheKine™ Ca2⁺/Mg2⁺-ATPase Activity Colorimetric Assay Kit|
|Applications notes||The kit provides a simple method for detecting Ca2⁺/Mg2⁺-ATPase activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, Ca2⁺/Mg2⁺-ATPase catalyzes ATP hydrolysis to produce ADP and inorganic phosphorus. The content of inorganic phosphorus can reflect the activity of ATPase.|
|Kit components||• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV • Reagent V
• Reagent VI
• Reagent VII
|Features & Benefits||• Determination of Ca2⁺/Mg2⁺-ATPase activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
|Usage notes||• 96 Well Clear Flat Bottom Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
• The test tube used in the determination is strictly phosphorus-free. Avoiding phosphorus contamination is the key to the success of detection.
|Storage instructions||Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The Ca2⁺/Mg2⁺-ATPase is an enzyme widely distributed in biomembrane system of the organism. It performs several functions in cell physiology and it can catalyze ATP hydrolysis to produce ADP and inorganic phosphorus.|
Fig. CheKine™ Ca2⁺/Mg2⁺-ATPase Activity Colorimetric Assay Kit
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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