Product Information

• Product name

  CheKine™ Blood Calcium Colorimetric Assay Kit

• Application notes

  The kit provides a simple method for detecting free calcium in the blood. In the assay, free calcium in the plasma reacts with GBHA in a strong alkaline solution to form a red calcium-GBHA complex which has an absorption peak at 520 nm; The free calcium concentration is calculated by measuring the absorbance at 520 nm.

Fig. CheKine™ Blood Calcium Colorimetric Assay Kit

Product Properties

• Kit components

  • Assay Buffer • Substrate • Standard

• Features & Benefits

  • Determination of free calcium in the blood. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

• Usage notes

  • Do not mix or substitute reagents or materials from other kit lots or vendors.
  • Avoid foaming or bubbles when mixing or reconstituting components.
  •Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
  • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
  •Blood should be collected on an empty stomach in the morning. It is advisable to perform serum potassium determination as soon as possible after blood sampling. Otherwise it will affect the accuracy of measurement results.
  • Reagent l is a self-prepared reagent, mixed according to the ratio of acetone and anhydrous methanol 1:9.
  •Observe good laboratory practices. Gloves, lab coat, and protective eyewear should always be worn. Never pipet by mouth. Do not eat, drink or smoke in the laboratory areas.

• Storage instructions

  Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  Almost all blood calcium exists in plasma, so blood calcium mainly refers to plasma calcium. Plasma calcium has two forms: ionized calcium and bound calcium, of which only ionized calcium directly plays a physiological role. It is in dynamic balance with bound calcium and is affected by blood pH. Blood calcium level is related to many important physiological functions, too high or too low will affect normal physiological functions.

• Alternative names

  Calcium; Blood Calcium; Ca2+