- Product name
CheKine™ Blood Calcium Colorimetric Assay Kit
- Application notes
The kit provides a simple method for detecting free calcium in the blood. In the assay, free calcium in the plasma reacts with GBHA in a strong alkaline solution to form a red calcium-GBHA complex which has an absorption peak at 520 nm; The free calcium concentration is calculated by measuring the absorbance at 520 nm.
- Kit components
• Assay Buffer • Substrate • Standard
- Features & Benefits
• Determination of free calcium in the blood. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.
- Usage notes
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
•Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
•Blood should be collected on an empty stomach in the morning. It is advisable to perform serum potassium determination as soon as possible after blood sampling. Otherwise it will affect the accuracy of measurement results.
• Reagent l is a self-prepared reagent, mixed according to the ratio of acetone and anhydrous methanol 1:9.
•Observe good laboratory practices. Gloves, lab coat, and protective eyewear should always be worn. Never pipet by mouth. Do not eat, drink or smoke in the laboratory areas.
- Storage instructions
Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Almost all blood calcium exists in plasma, so blood calcium mainly refers to plasma calcium. Plasma calcium has two forms: ionized calcium and bound calcium, of which only ionized calcium directly plays a physiological role. It is in dynamic balance with bound calcium and is affected by blood pH. Blood calcium level is related to many important physiological functions, too high or too low will affect normal physiological functions.
- Alternative names
Calcium; Blood Calcium; Ca2+
Most popular with customers
CheKine™ Blood Calcium Colorimetric Assay Kit
Click to download booklet for more details
Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact email@example.com for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.