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CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit

Product Information

  • Product name

    CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit

  • Application notes

    The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of α-ketoglutarate and alanine at 37°C and pH7.4 to produce glutamic acid and pyruvate; pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.

Fig. CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit

Product Properties

  • Kit components

    • Extraction Buffer
    • Reagent I
    • Reagent II
    • Reagent III
    • Standard

  • Features & Benefits

    • Determination of ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
    • Detailed sample preparation and result calculation methods are provided.
    • Simple operation steps.
    • Standard and standard curve line are provided.

  • Usage notes

    • Do not mix or substitute reagents or materials from other kit lots or vendors.
    • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
    • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.

  • Storage instructions

    Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Alanine Aminotransferase (ALT/GPT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum ALT activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, ALT has the highest content in cardiomyocytes, and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice.

  • Alternative names

    ALT; GPT; Alanine aminotransferase; glutamate pyruvic transaminase

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CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTB1410

  • Size
  • Price
  • 96T $40

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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