|Product name||Cell Proliferation Assay Cocktail|
|Applications notes||Cell Proliferation Assay Cocktail can not only accurately detect cell proliferation status directly from DNA level, but also realize high-throughput detection of cell proliferation status through cell metabolism level. It is a universal cell proliferation detection product.|
•EdU Cell Proliferation Image Kit (Green Fluorescence)-100T
EdU (10 mM)
BSA Wash Solution (5×)
AbFluor 488 azide
•Cell Counting Kit-8 (CCK-8)-2000T
|Features & Benefits||• Standardized: The patented cytotoxicity positive reference substance can perfectly solve the problem of non-standardization of proliferation/toxicity test results at present.
• Universal: It can detect cell proliferation status most directly and accurately, and it can be also chosen as high-throughput detection detection, which covers most cell proliferation/toxicity detection needs.
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Cell proliferation is a process of increasing the number of cells by means of cell division, which will produce many important changes, including the synthesis of DNA, the increase of cell metabolism, the expression of proliferation-specific proteins and so on.|
Fig.1. HEK293 and L929 cells in the positive control group were induced by cytotoxicity inducer for 30 min, while the control group was not given any treatment. Enhanced Cell Counting Kit-8 (CCK-8) was used to detect the cytotoxicity of HEK293 and L929 cells after cytotoxicity induction.
Fig.2. Cell Proliferation EdU Image Kit (Green Fluorescence) was used to detect the cytotoxicity of HEK293 cells after cytotoxicity induction.
EDU is suitable for the detection of cell proliferation. If you want to do tissue staining, it is recommended to use proliferative antibody PCNA (Cat.no ABP0112, ABL1040) or direct labeled antibody for IHC test.
EDU is used for labelling and quantification of nascent cells, while CCK8 is suitable for cell proliferation toxicity and drug screening. Choose according to actual needs.
It is best to observe it on the same day. If it cannot be observed, it is recommended to add an anti-fluorescence quencher and store it in the dark at 4 °C.
Yes, generally it is recommended to be around 70%, the cells should not be too dense, and it needs to be adjusted appropriately according to the cell type.
Yes，it can be used for flow testing.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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