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Catenin-β (phospho Ser37) Polyclonal Antibody

Catenin-β (phospho Ser37) Polyclonal Antibody

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Specification

Product name Catenin-β (phospho Ser37) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Catenin-β around the phosphorylation site of S37
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-catenin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 85 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Catenin beta 1 encoded by CTNNB1 is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Catenin beta 1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, catenin beta 1 binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in CTNNB1 are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for CTNNB1.
Gene ID 1499
Alternative CTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-catenin
Others Phospho-Catenin-β (S37) Polyclonal Antibody detects endogenous levels of Catenin-β protein only when phosphorylated at S37.
Accession P35222

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-Catenin-β (S37) Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using Phospho-Catenin-β (S37) Polyclonal Antibody diluted at 1:2000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue. 1, Catenin-β (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue. 1, Catenin-β (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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