|Product name||Caspase-3 Mouse Monoclonal Antibody (4B8)|
|Immunogen||Synthetic Peptide of Caspase-3 at AA range of 20-100|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:200).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen|
|Storage buffer||PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||CASP3 (caspase 3) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein.|
|Others||Caspase-3 protein detects endogenous levels of CASP3.|
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Caspase-3 Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Prostate Tissue using Caspase-3 Mouse mAb diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse LiverTissue using Caspase-3 Mouse mAb diluted at 1:200.
Author：X Rui, Y Yang, J Wu Publication name：Photodiagnosis and Photodynamic Therapy IF：2.75