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Casein Kinase IIα (phospho Tyr255) Polyclonal Antibody

Casein Kinase IIα (phospho Tyr255) Polyclonal Antibody

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Specification

Product name Casein Kinase IIα (phospho Tyr255) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Casein Kinase IIα around the phosphorylation site of Y255
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CSNK2A1; CK2A1; Casein kinase II subunit alpha; CK II alpha

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
Gene ID 1457
Alternative CSNK2A1; CK2A1; Casein kinase II subunit alpha; CK II alpha
Others Phospho-Casein Kinase IIα (Y255) Polyclonal Antibody detects endogenous levels of Casein Kinase IIα protein only when phosphorylated at Y255.
Accession P68400
Observed Band(KD) 45

Image & description

Fig. Western Blot analysis of HeLa cells using Phospho-Casein Kinase IIα (Y255) Polyclonal Antibody.

Fig. Western Blot analysis of HeLa cells using Phospho-Casein Kinase IIα (Y255) Polyclonal Antibody.

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