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Casein Kinase Iα Polyclonal Antibody

Casein Kinase Iα Polyclonal Antibody

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Specification

Product name Casein Kinase Iα Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Casein Kinase Iα at AA range: 100-180
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Iε, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair, membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Casein kinase I isoform alpha-like (CSNK1A1L) is a 337 amino acid protein that shares a high degree of sequence similarity with the alpha isoform of casein kinase 1. CSNK1A1L resides in the cytoplasm and participates in the Wnt signaling pathway. By utilizing ATP within its protein kinase domain, CSNK1A1L phosphorylates a large number of proteins.
Gene ID 1452
Alternative CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1
Others Casein Kinase Iα Polyclonal Antibody detects endogenous levels of Casein Kinase Iα protein.
Accession P48729
Observed Band(KD) 35

Image & description

Fig. Western Blot analysis of various cells using Casein Kinase Iα Polyclonal Antibody.

Fig. Western Blot analysis of various cells using Casein Kinase Iα Polyclonal Antibody.

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