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CaMKIIα/β/δ (phospho Thr305) Polyclonal Antibody

CaMKIIα/β/δ (phospho Thr305) Polyclonal Antibody

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Specification

Product name CaMKIIα/β/δ (phospho Thr305) Polyclonal Antibody
Immunogen Synthesized peptide derived from human CaMKIIα/β/δ around the phosphorylation site of T305
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Calcium/calmodulin dependent protein kinase II beta belongs to the serine/threonine protein kinase family and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of CAMK2B is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.
Gene ID 816/817
Alternative CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca
Others Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody detects endogenous levels of CaMKIIα/β/δ protein only when phosphorylated at T305.
Accession Q9UQM7/Q13554/Q13557
Observed Band(KD) 54

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 453 cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 453 cells using Phospho-CaMKIIα/β/δ (T305) Polyclonal Antibody diluted at 1:1000.

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