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BRCA1 Polyclonal Antibody

BRCA1 Polyclonal Antibody

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Specification

Product name BRCA1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of S1524
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative BRCA1; RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background BRCA1encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein (BRCA1, DNA repair associated) combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
Gene ID 672
Alternative BRCA1; RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53
Others BRCA1 Polyclonal Antibody detects endogenous levels of BRCA1 protein.
Accession P38398
Observed Band(KD) 200

Image & description

Fig.1. Western Blot analysis of various cells using BRCA1 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using BRCA1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293 cells using BRCA1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293 cells using BRCA1 Polyclonal Antibody diluted at 1:1000.

Fig.3. Western Blot analysis of JK using BRCA1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Fig.3. Western Blot analysis of JK using BRCA1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

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