|Product name||ATM Mouse Monoclonal Antibody (1D1)|
|Immunogen||Recombinant Protein of ATM of ATM|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:200).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen|
|Storage buffer||PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The protein encoded by ATM (ATM serine/threonine kinase) belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in ATM are associated with ataxia telangiectasia, an autosomal recessive disorder.|
|Others||ATM protein detects endogenous levels of ATM.|
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using ATM Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATM Mouse mAb diluted at 1:200.