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Anti-TBP/TATA Binding Protein Monoclonal Antibody (2C6)

Anti-TBP/TATA Binding Protein Monoclonal Antibody (2C6)

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Specification

Product name Anti-TBP/TATA Binding Protein Monoclonal Antibody (2C6)
Immunogen Recombinant Protein
Host Mouse
Reactivity Human, Mouse, Rat
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Gene ID 6908
Alternative TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit
Accession P20226
Observed Band(KD) 35-45

Image & description

Fig. Western blot analysis of TATA Binding Protein express in  Hela (lane A), Mouse brain tissue (lane B) and Rat brain tissue (lane C) with TBP/TATA Binding Protein monoclonal antibody with 1:2000 dilution.

Fig. Western blot analysis of TATA Binding Protein express in Hela (lane A), Mouse brain tissue (lane B) and Rat brain tissue (lane C) with TBP/TATA Binding Protein monoclonal antibody with 1:2000 dilution.

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