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Anti-S Tag Mouse Monoclonal Antibody (9T10)

Anti-S Tag Mouse Monoclonal Antibody (9T10)

Views(91) Publications(1) Catalog no(A02130)
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Specification

Product name Anti-S Tag Mouse Monoclonal Antibody (9T10)
Immunogen Synthetic Peptide
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative S tag; KETAAAKFERQHMDS tag; S peptide; S peptide epitope

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acids KETAAAKFERQHMDS. S Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.
Alternative S tag; KETAAAKFERQHMDS tag; S peptide; S peptide epitope

Image & description

Fig. Western blot analysis of 1ug S-Tag fusion protein with Anti-S Tag monoclonal antibody in 1:2000 (lane A) , 1:5000 (lane B) dilutions.

Fig. Western blot analysis of 1ug S-Tag fusion protein with Anti-S Tag monoclonal antibody in 1:2000 (lane A) , 1:5000 (lane B) dilutions.

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