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Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

Product Information

  • Product name

    Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

  • Immunogen

    Recombinant Protein

  • Host


  • Reactivity

    Arabidopsis, Brassica napus, Corn

  • Applications


  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).

  • Clonality


  • Isotype

    Mouse IgG1

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig. Western blot analysis (1:3000) of plant Actin expression in Arabidopsis (lane A) and Nicotiana tabacum (lane B) with Anti-Plant Actin Mouse Monoclonal Antibody (3T3).

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.

  • Gene ID


  • Alternative names

    AT3G12110; actin-11

  • Accession


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis)


  2. Phosphorylation-mediated dynamics of nitrate transceptor NRT1. 1 regulate auxin flux and nitrate signaling in lateral root growth


  3. EMS1 and BRI1 control separate biological processes via extracellular domain diversity and intracellular domain conservation

    B Zheng, Q Bai. Nature Communications

  4. Retardation of the Calvin cycle contributes to the reduced CO2 assimilation ability of rice stripe virus-infected N. benthamiana and suppresses viral infection

    J Bi, Y Yang, B Chen, J Zhao, Z Chen, B Song. Front. Microbiol., 20 March 2019.

  5. Suppression of auxin signalling promotes rice susceptibility to Rice black streaked dwarf virus infection

    H Zhang, X Tan, L Li, Y He, G Hong, J Li. Molecular plant pathology, 2019.

  6. Rice black‐streaked dwarf virus P10 acts as either a synergistic or antagonistic determinant during superinfection with related or unrelated virus

    H Zhang, X Tan, Y He, K Xie, L Li. Molecular plant pathology, 2019.

  7. Suppression of auxin signalling promotes rice susceptibility to Rice black streaked dwarf virus infection

    Hehong Zhang, Xiaoxiang Tan, Lulu Li, et al. Mol Plant Pathol. 2019 Jun 27.

  8. Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis)

    Shenling Peng, Shengnan Huang, Zhiyong Liu and Hui Feng. Int J Mol Sci. 2019 May 10;20(9). pii: E2310.

  9. Downregulation of Nuclear Protein H2B Induces Salicylic Acid Mediated Defense Against PVX Infection in Nicotiana benthamiana

    Xue Yang, Yuwen Lu, Xing Zhao, et al. Front Microbiol. 2019 May 8;10:1000.

  10. The Dual Effect of the Brassinosteroid Pathway on Rice Black-Streaked Dwarf Virus Infection by Modulating the Peroxidase-Mediated Oxidative Burst and Plant Defense

    Hehong Zhang, Yuqing He, Xiaoxiang Tan, et al. Mol Plant Microbe Interact. 2019 Apr 15:MPMI10180285R.

  11. Rice black‐streaked dwarf virus P10 acts as either a synergistic or antagonistic determinant during superinfection with related or unrelated virus

    Hehong Zhang, Xiaoxiang Tan, Yuqing He, et al. molecular plant pathology

  12. Tissue-specific accumulation and subcellular localization of chalcone isomerase (CHI) in grapevine

    Huiling Wang, Wei Wang, Jicheng Zhan, et al. Plant Cell, Tissue and Organ Culture (PCTOC)

  13. Hyper-acidic fusion minipeptides escort the intrinsic antioxidative ability of the pattern recognition receptor CRP in non-animal organisms

    Mengru Zhang, Yanjuan Liu, Zhibin Liu, et al. Sci Rep. 2019 Feb 28;9(1):3032.

  14. The β5 subunit is essential for intact 26S proteasome assembly to specifically promote plant autotrophic growth under salt stress

    Jia‐Jia Han, Xiaoyuan Yang, et al. New Phytologist. (2018).

  15. Bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice

    Guo J, Xu C, Wu D, et al. Nat Genet. 2018, 50(2): 297-306.

  16. Immunoblotting Quantification Approach for Identifying Potential Hypoallergenic Citrus Cultivars

    Wu J, Deng W, Lin D, et al. J Agric Food Chem. 2018, 66(8): 1964-1973.

  17. Role of Cleavage and Polyadenylation Specificity Factor 100: Anchoring Poly (A) Sites and Modulating Transcription Termination

    Lin, Juncheng, et al. The Plant Journal (2017).

  18. The Arabidopsis F-box E3 ligase RIFP1 plays a negative role in abscisic acid signalling by facilitating ABA receptor RCAR3 degradation.

    Li Y, Zhang L, Li D, et al. Plant, cell & environment, 2016, 39(3): 571-582.

  19. The Protein Elicitor PevD1 Enhances Resistance to Pathogens and Promotes Growth in Arabidopsis.

    Liu M, Khan N U, Wang N, et al. International Journal of Biological Sciences, 2016, 12(8): 931.

  20. Fungal cellulase is an elicitor but its enzymatic activity is not required for its elicitor activity

    Ma Y, Han C, Chen J, et al. Molecular plant pathology, 2015, 16(1): 14-26.

Cat #: A01050

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

Contact information

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