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Anti-Myc Tag Mouse Monoclonal Antibody (2D5)

Product Information

  • Product name

    Anti-Myc Tag Mouse Monoclonal Antibody (2D5)

  • Immunogen

    Synthetic Peptide

  • Host


  • Applications

    IF, IP, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1000), IP (1:200).

  • Clonality


  • Isotype

    Mouse IgG

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Immunofluorescence staining (1:2000) of Myc fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2. Western blot analysis of 1ug Myc fusion protein with Anti-Myc Mouse Monoclonal Antibody (2D5) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).

Fig.3. IP (1:200)-WB (1:5000) analysis of Myc fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Myc-tag protein (lane B); IP transfected 293 cell lysate with normal Mouse IgG (lane C) or with Anti Myc tag mAb (lane D) , and without both normal Mouse IgG and Myc tag mAb (lane E).

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

  • Alternative names

    Myc tag; Myc Epitope; c-Myc; bHLHe39; myc proto-oncogene protein; oncogene c-Myc


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. LIN28A activates the transcription of NANOG in dairy goat male germline stem cells

    F Ma, X Du, Y Wei, Z Zhou, DZJ Clotaire. Journal of cellular physiology, 2019.

  2. TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis

    Jinyuan Cui, Jia Hu, Zhilan Ye, et al. Science Bulletin Available online 25 May 2019

  3. LIN28A activates the transcription of NANOG in dairy goat male germline stem cells

    Fanglin Ma, Xiaomin Du, Yudong Wei, et al. J Cell Physiol. 2019 Jun;234(6):8113-8121.

  4. Classical swine fever virus nonstructural protein p7 modulates infectious virus production

    Zhao C, Shen X, Wu R, et al. Sci Rep, 2017, 7(1): 12995.

  5. Abnormal Ergosterol Biosynthesis Activates Transcriptional Responses to Antifungal Azoles

    Hu C, Zhou M, Wang W, et al. Front Microbiol, 2018, 9: 9.

  6. Smurf1 restricts the antiviral function mediated by USP25 through promoting its ubiquitination and degradation

    Qian G, Hu X, Li G, et al. Biochem Biophys Res Commun, 2018, 6; 498(3): 537-543.

  7. FBW7 loss promotes epithelial-to-mesenchymal transition in non-small cell lung cancer through the stabilization of Snail protein

    Zhang Y, Zhang X X, Ye M X, et al. Cancer letters, 2018, 419: 75-83.

  8. The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

    Zheng, Liming, et al. Scientific Reports 6 (2016).

Cat #: A02060

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

Contact information

Do not hesitate to contact us if you have any questions.

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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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