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Anti-MBP Tag Mouse Monoclonal Antibody (9Y5)

Anti-MBP Tag Mouse Monoclonal Antibody (9Y5)

Views(133) Publications(0) Catalog no(A02070)
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Specification

Product name Anti-MBP Tag Mouse Monoclonal Antibody (9Y5)
Immunogen Recombinant Protein
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; MMBP; Periplasmic maltose binding protein

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Maltose Binding Protein, also called MBP, is a member of the maltose/maltodextrin system of E.coli which is accountable for the uptake and efficient catabolism of maltodextrins. Maltose binding protein is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies).
Alternative ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; MMBP; Periplasmic maltose binding protein

Image & description

Fig. Western blot analysis of 0.5ug MBP fusion protein with Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) in 1:2000 (lane A), 1:3000 (lane B) and 1:5000 (lane C) dilutions.

Fig. Western blot analysis of 0.5ug MBP fusion protein with Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) in 1:2000 (lane A), 1:3000 (lane B) and 1:5000 (lane C) dilutions.

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