Product Information

• Product name

  Anti-MBP Tag Mouse Monoclonal Antibody (9Y5)

• Immunogen

  Recombinant Protein

• Host

  Mouse

• Applications

  WB

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:5000.

• Clonality

  Monoclonal

• Isotype

  Mouse IgG

• Purification

  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Fig.Western blot analysis of 0.5ug MBP fusion protein with Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) in 1:2,000 (lane A), 1:3,000 (lane B) and 1:5,000 (lane C) dilutions.

Product Properties

• Formulation

  Liquid solution

• Storage buffer

  Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  Maltose Binding Protein, also called MBP, is a member of the maltose/maltodextrin system of E.coli which is accountable for the uptake and efficient catabolism of maltodextrins. Maltose binding protein is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies).

• Alternative names

  ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; MMBP; Periplasmic maltose binding protein