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Anti-KT3 Tag Mouse Monoclonal Antibody (14D8)

Anti-KT3 Tag Mouse Monoclonal Antibody (14D8)

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Specification

Product name Anti-KT3 Tag Mouse Monoclonal Antibody (14D8)
Immunogen Synthetic Peptide
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative KT3; KPPTPPPEPET Epitope; KPPTPPPEPET tag; KT3 epitope

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. KT3 epitope tag can be used to monitor expression of the protein products transfected with cDNA constructs. The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen.
Alternative KT3; KPPTPPPEPET Epitope; KPPTPPPEPET tag; KT3 epitope

Image & description

Fig. Western blot analysis of 1ug KT3 fusion protein with Anti-KT3 monoclonal antibody in 1:3000 (lane A) and 1:5000 (lane B) dilutions.

Fig. Western blot analysis of 1ug KT3 fusion protein with Anti-KT3 monoclonal antibody in 1:3000 (lane A) and 1:5000 (lane B) dilutions.

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