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Anti-His Tag Mouse Monoclonal Antibody (5C3)

Product Information

  • Product name

    Anti-His Tag Mouse Monoclonal Antibody (5C3)

  • Immunogen

    Synthetic Peptide

  • Host


  • Applications

    IF, IP, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1500), IP (1:300).

  • Clonality


  • Isotype

    Mouse IgG

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).

Fig.3. IP (1:300) - WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).

Product Properties

  • Formulation

    Liquid solution

  • Concentration

    1 mg/ml

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

  • Alternative names

    His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Nerve growth factor and Tropomyosin receptor kinase A are increased in the gastric mucosa of patients with functional dyspepsia

    H Shi, S Zhu, B Qin. BMC Gastroenterology, 2019 - Springer

  2. Dendritic cells targeting the Lactobacillus plantarum NC8 strain with a surface-displayed single-chain variable fragment of CD11c induce an antigen-specific protective cellular immune response

    Jing Liu, Guilian Yang. Life Sciences

  3. A novel Cre recombinase-mediated in vivo minicircle DNA (CRIM) vaccine provides partial protection against Newcastle disease virus


  4. The florigen interactor BdES43 represses flowering in the model temperate grass Brachypodium distachyon

    S Cao, X Luo. The Plant Journal

  5. Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat

    T Qi, J Guo, P Liu. Molecular Plant

  6. Regulation of resveratrol O‐methyltransferase gene in pterostilbene defensing the sour rot of wine grape

    X Ren, X Zhang, X Zhai, R Deng. Journal of food Biochemistry,2019.

  7. Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL‐18 delivered by Salmonella

    X Gao, K Xu, G Yang, C Shi, H Huang. Journal of applied microbiology, 2019.

  8. Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice

    HM Zakria, B Han, F Yue, L Mu, Y Fang, X Li. Biomedicine & Pharmacotherapy, 2019.

  9. A Novel Cre Recombinase-Mediated In Vivo Minicircle DNA (CRIM) Vaccine Provides Partial Protection against Newcastle Disease Virus

    Yanlong Jiang, Xing Gao, Ke Xu, et al. Appl Environ Microbiol. 2019 Jul 1;85(14). pii: e00407-19.

  10. Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL‐18 delivered by Salmonella

    Xing Gao, Ke Xu, Guilian Yang, et al. J Appl Microbiol. 2019 May;126(5):1362-1372.

  11. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus

    Baoquan Han, Kun Xu, et al. Fish & Shellfish Immunology Volume 84, January 2019, Pages 948-954

  12. Zebrafish intelectin 1 (zITLN1) plays a role in the innate immune response

    LeiChen, JieYan, et al. Fish & Shellfish Immunology. 2018; 83: 96-103.

  13. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infectionon Carassius auratus.

    Baoquan Han, Kun Xu, et al. Fish & Shellfish Immunology. 2018; 84: 948-954.

  14. A zebrafish intelectin ortholog agglutinates both Gram-negative and Gram-positive bacteria with binding capacity to bacterial polysaccharide

    Chen, Lei, et al. Fish & shellfish immunology 55 (2016): 729-736.

  15. An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A

    Liu J, Gao S, Kang L, et al. Nanoscale Res Lett, 2017, 12(1): 227.

  16. An Asparagine-Rich Protein Nbnrp1 Modulate Verticillium dahliae Protein PevD1-Induced Cell Death and Disease Resistance in Nicotiana benthamiana

    Liang Y, Cui S, Tang X, et al. Front Plant Sci, 2018, 7; 9: 303.

  17. MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells

    Wan, Quanyuan, et al. Frontiers in Immunology 8 (2017): 189.

  18. Evidence of VP1 of duck hepatitis A type 1 virus as a target of neutralizing antibodies and involving receptor-binding activity

    Li, Xiaojun, et al. Virus Research 227 (2017): 240-244.

  19. Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis

    Lv, Meinan, et al. Journal of the American Chemical Society 138.20 (2016): 6427-6435.

  20. Construction of a directional T vector for cloning PCR products and expression in Escherichia coli.

    Liang X Y, Liang Z C, Zhang Z, et al. Plasmid, 2015, 79: 15-21.

  21. Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2.

    Zhu M, Hu Y, Li G, et al. Nanoscale research letters, 2014, 9(1): 1.

Cat #: A02050

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

Contact information

Do not hesitate to contact us if you have any questions.

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