Product Information

• Product name

  Anti-HA Tag Mouse Monoclonal Antibody (4F6)

• Immunogen

  Synthetic Peptide

• Host

  Mouse

• Applications

  IF, IP, WB

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:5000, IF: 1:1000, IP: 1:200.

• Clonality

  Monoclonal

• Isotype

  Mouse IgG

• Purification

  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Fig.1.Immunofluorescence staining (1:2,000) of HA fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2.IP (1:200) - WB (1:5,000) analysis of HA fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with HA-tag protein (lane B); IP untransfected 293 cell lysate with Anti HA tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D), or with Anti HA tag mAb (lane E), and IP transfected 293 without both normal Mouse IgG and HA tag mAb (lane F).

Product Properties

• Formulation

  Liquid solution

• Storage buffer

  Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  Human influenza hemagglutinin(HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.

• Alternative names

  HA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain