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Anti-GST Tag Mouse Monoclonal Antibody (2A8)

Product Information

  • Product name

    Anti-GST Tag Mouse Monoclonal Antibody (2A8)

  • Immunogen

    Recombinant Protein

  • Host


  • Applications


  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).

  • Clonality


  • Isotype

    Mouse IgG

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig. Western blot analysis of GST fusion protein with Anti-GST Mouse Monoclonal Antibody (2A8) in 1:5000 dilutions (lane A) and 1:10000 dilutions (lane A) with 0.5ug GST fusion protein, separately.

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins.

  • Alternative names

    GST; Glutathione S Transferase; Glutathione S-transferase class-mu 26 kDa isozyme; Sj26 antigen; sjGST


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. The polymeric immunoglobulin receptor-like protein from Marsupenaeus japonicus is a receptor for white spot syndrome virus infection

    GJ Niu. PLoS Pathogens

  2. Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat

    T Qi, J Guo, P Liu. Molecular Plant

  3. Cross-microbial protection via priming a conserved immune co-receptor through juxtamembrane phosphorylation in plants

    BQ Gong, J Guo, N Zhang. Cell Host & Microbe

  4. The polymeric immunoglobulin receptor-like protein from Marsupenaeus japonicus is a receptor for white spot syndrome virus infection

    Guo-Juan Niu, Shuai Wang, Ji-Dong Xu, et al. PLoS Pathog. 2019 Feb 6;15(2):e1007558.

  5. Interaction of porcine reproductive and respiratory syndrome virus proteins with SUMO-conjugating enzyme reveals the SUMOylation of nucleocapsid protein

    Wang C, Zeng N, Liu S, et al. PLoS One, 2017, 12(12): e0189191.

  6. An Asparagine-Rich Protein Nbnrp1 Modulate Verticillium dahliae Protein PevD2-Induced Cell Death and Disease Resistance in Nicotiana benthamiana

    Liang Y, Cui S, Tang X, et al. Front Plant Sci, 2018, 7; 9: 304.

  7. Antimicrobial activity of a newly identified Kazal-type serine proteinase inhibitor, CcKPI1, from the jellyfish Cyanea capillata

    Zhou Y, Liu G, Cheng X, et al.

  8. Molecular cloning, prokaryotic expression and promoter analysis of squalene synthase gene from Schizochytrium Limacinum.

    Zhu L, Zhang X, Chang L, et al. Applied biochemistry and microbiology, 2014, 50(4): 411-419.

Cat #: A02030

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer:
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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