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Anti-GST Tag Mouse Monoclonal Antibody (2A8)

Anti-GST Tag Mouse Monoclonal Antibody (2A8)

Views(130) Publications(5) Catalog no(A02030)
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Specification

Product name Anti-GST Tag Mouse Monoclonal Antibody (2A8)
Immunogen Recombinant Protein
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative GST; Glutathione S Transferase; Glutathione S-transferase class-mu 26 kDa isozyme; Sj26 antigen; sjGST

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins.
Alternative GST; Glutathione S Transferase; Glutathione S-transferase class-mu 26 kDa isozyme; Sj26 antigen; sjGST

Image & description

Fig. Western blot analysis of GST fusion protein with Anti-GST Mouse Monoclonal Antibody (2A8) in 1:5000 dilutions (lane A) and 1:10000 dilutions (lane A) with 0.5ug GST fusion protein, separately.

Fig. Western blot analysis of GST fusion protein with Anti-GST Mouse Monoclonal Antibody (2A8) in 1:5000 dilutions (lane A) and 1:10000 dilutions (lane A) with 0.5ug GST fusion protein, separately.

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