- Product name
Anti-GFP Tag Mouse Monoclonal Antibody (3D3)
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IP (1:200).
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.
- Alternative names
GFP; Green fluorescent protein
Most popular with customers
Application: IF, IP, WB
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Disrupting the association of Autographa californica multiple nucleopolyhedrovirus Ac93 with cellular ESCRT-III/Vps4 hinders nuclear egress of nucleocapsids and intranuclear microvesicles formation
T Liu, Y Li, B Qiao. VIROLOGY
Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat
T Qi, J Guo, P Liu. Molecular Plant
Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer׳s disease
Qian Guo, Xiaoyao Zheng, Peng Yang, et al. Acta Pharm Sin B. 2019 May;9(3):590-603.
Effects of Long Form of CAPON Overexpression on Glioma Cell Proliferation are Dependent on AKT/mTOR/P53 Signaling
Dong Liang, Yunnong Song, Guangwei Fan, et al. Int J Med Sci. 2019 Apr 25;16(4):614-622.
DNA methylation and hydroxymethylation are associated with the degree of coronary atherosclerosis in elderly patients with coronary heart disease
Dan Jiang, Min Sun, Linna You, et al. Life Sci. 2019 May 1;224:241-248.
ISG12a and its interaction partner NR4A1 are involved in TRAIL‐induced apoptosis in hepatoma cells
Nianli Liu, Zhiyuan Wu, Aoxing Chen, et al. J Cell Mol Med. 2019 Mar 1.
Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus
Baoquan Han, Kun Xu, et al. Fish & Shellfish Immunology Volume 84, January 2019, Pages 948-954
Establishment of insect cell lines expressing green fluorescent protein on cell surface based on AcMNPV GP64 membrane fusion characteristic
Ben-Xiang Qi, Ying-Jian Chen, et al. Cytotechnology. 2017; 69(5): 775-783.
Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infectionon Carassius auratus.
Baoquan Han, Kun Xu, et al. Fish & Shellfish Immunology. 2018; 84: 948-954.
Construction of the IRES-based Vector for Multiple Gene Co-expression
Tian C H, Xie X M, Li Y, et al. China Biotechnology , 2017, 37(7): 97-105.
E1A-engineered human umbilical cord mesenchymal stem cells as carriers and amplifiers for adenovirus suppress hepatocarcinoma in mice
Li, Zhenzhen, et al. Oncotarget 7.32 (2016): 51815.
Exogenous gene can be integrated into Nosema bombycis genome by mediating with a non-transposon vector.
Guo R, Cao G, Lu Y, et al. Parasitology research, 2016: 1-6.
Novel hypovirulence-associated RNA mycovirus in the plant-pathogenic fungus Botrytis cinerea: Molecular and biological characterization.
Yu L, Sang W, Wu M D, et al. Applied and environmental microbiology, 2015, 81(7): 2299-2310.