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Anti-GAPDH Rabbit Polyclonal Antibody

Anti-GAPDH Rabbit Polyclonal Antibody

Views(60) Publications(1) Catalog no(A01021)
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Specification

Product name Anti-GAPDH Rabbit Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Chicken, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xneous
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IHC-P (1:200).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background GAPDH encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. Glyceraldehyde-3-phosphate dehydrogenase has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Glyceraldehyde-3-phosphate dehydrogenase has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, Glyceraldehyde-3-phosphate dehydrogenase contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Gene ID 2597
Alternative GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Others The antibody detects endogenous GAPDH protein.
Accession P04406
Observed Band(KD) 37

Image & description

Fig. Western blot analysis of 293T (1), Rat brain (2), NIH 3T3 (3), Sheep Muscle (4), Rabbit testis (5), diluted at 1:20000. Secondary antibody was diluted at 1:20000.

Fig. Western blot analysis of 293T (1), Rat brain (2), NIH 3T3 (3), Sheep Muscle (4), Rabbit testis (5), diluted at 1:20000. Secondary antibody was diluted at 1:20000.

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