- Product name
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast
IF, IHC-p, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400).
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Fig.1. Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.5. Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
- Gene ID
- Alternative names
GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Most popular with customers
Application: IF, IHC-p, IP, WB
Reactivity: Human, Mouse, Rat, Yeast
Application: IF, IHC-p, WB
Reactivity: Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast
Application: IF, IHC-p, WB
Reactivity: Human, Mouse, Rat
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.
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L-Fucose ameliorates DSS-induced acute colitis via inhibiting macrophage M1 polarization and inhibiting NLRP3 inflammasome and NF-kB activation
Ruohang He, Ying Li, Chaoqun Han, et al. Int Immunopharmacol. 2019 May 24;73:379-388.
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Yan‑Zi Zhang, Xiao‑Lu Sui, Yun‑Peng Xu, et al. Int J Mol Med. 2019 Jul;44(1):172-184.
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Compound α-keto acid tablet supplementation alleviates chronic kidney disease progression via inhibition of the NF-kB and MAPK pathways
Meng Wang, Huzi Xu, Octavia Li‑Sien Chong Lee Shin, et al. J Transl Med. 2019 Apr 11;17(1):122.
Bone marrow-derived Ly6C− macrophages promote ischemia-induced chronic kidney disease
Qian Yang, Yuxi Wang, Guangchang Pei, et al. Cell Death Dis. 2019 Mar 29;10(4):291.
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Erhui Jiang, Zhi Xu, Meng Wang, et al. FASEB J. 2019 Apr;33(4):5690-5703.
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Yuan Liu, Aiping Cao, et al. BMC Infectious Diseases. 2017; 17(1): 403.
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Sen Yang, Ju He, et al. Experimental and Therapeutic Medicine. 2018; 16(5): 4042-4048.
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Fengming Zhu, Ying Yao, et al. American Journal of Translational Research. 2017; 9(4): 1694-1707.
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Yuan-Feng Zeng, Yi-Sheng Xiao, et al. World Journal of Gastroenterology. 2018; 24(34): 3884-3897.
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Liang Y, Yi L, Liu P, et al. Journal of Cancer. 2018; 9(19): 3603-3612.
Adipose-derived mesenchymal stem cells employed exosomes to attenuate AKI-CKD transition through tubular epithelial cell dependent Sox9 activation
Zhu F, Chong Lee Shin OLS, Pei G, et al. Oncotarget, 2017, 8(41): 70707-70726.
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Wang S, Song Y, Wang Y, et al. Chemical Research in Chinese Universities, 2018, 34(1): 67–74.
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Liu H, Liu Y, Li Y, et al. Exp Ther Med, 2018, 15(3): 2525-2532.
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Ren L, Wang P, Wang Z, et al. Mol Med Rep,16(4): 4334-4340.
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Zhang L, Song J, Bai T, et al. Sci Rep, 2017, 10; 7(1): 4950.
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Gong J, Han J, He J, et al. Lab Invest, 97(9): 1020-1032.
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Niu, Xuan, et al. Arteriosclerosis, Thrombosis, and Vascular Biology (2017): ATVBAHA-116.
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Chen, Xu, et al. PloS one 12.2 (2017): e0172744.
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Liu, G, et al. Gene 580.1(2016): 47-57.
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Han, Juan, et al. Journal of Leukocyte Biology (2016): jlb-2A0116.
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Zuo Y, Xiong N, Zhao H. Journal of Huazhong University of Science and Technology [Medical Sciences], 2016, 36(4): 548-553.
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Zhang L, Song J, Bai T, et al. European journal of pharmacology, 2016, 780: 180-187.
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Liu Y, Feng X, Zhang Y, et al. Journal of Orthopaedic Research, 2016.
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Zeng T, Zhou J, He L, et al. PloS one, 2016, 11(3): e0149677.
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Zheng X X, Li X Y, Lyu Y N, et al. Experimental physiology, 2016, 101(2): 260-271.
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Liu W, Wang H, Wang Y, et al. Psychiatry research, 2015, 228(3): 257-264.
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Zeng T, Liu F, Zhou J, et al. Endocrine journal, 2015 (0).
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Wang J H, Zhou W W, Cheng S T, et al. Molecular medicine reports, 2015, 12(2): 1810-1816.
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Zeng Y F, Xiao Y S, Lu M Z, et al. Experimental and molecular pathology, 2015, 98(2): 260-267.
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Wang J H, Zheng W W, Cheng S T, et al. Molecular medicine reports, 2015, 11(6): 4220-4224.
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Zheng X, Li X, Lyu Y, et al. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 2016, 22: 2751.