- Product name
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast
IF, IHC, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IHC (1:200).
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
- Gene ID
- Alternative names
GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Most popular with customers
Application: IF, IHC, WB
Reactivity: Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast
Application: IF, IHC, WB
Reactivity: Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
Application: IF, IHC, IP, WB
Reactivity: Human, Mouse, Rat, Yeast
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Adipose-derived mesenchymal stem cells employed exosomes to attenuate AKI-CKD transition through tubular epithelial cell dependent Sox9 activation
Zhu F, Chong Lee Shin OLS, Pei G, et al. Oncotarget, 2017, 8(41): 70707-70726.
Design and synthesis of novel bispecific molecules for inducing BRD4 protein degradation
Wang S, Song Y, Wang Y, et al. Chemical Research in Chinese Universities, 2018, 34(1): 67–74.
Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria
Liu H, Liu Y, Li Y, et al. Exp Ther Med, 2018, 15(3): 2525-2532.
Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro
Ren L, Wang P, Wang Z, et al. Mol Med Rep,16(4): 4334-4340.
Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2
Zhang L, Song J, Bai T, et al. Sci Rep, 2017, 10; 7(1): 4950.
Paired related homeobox protein 1 regulates PDGF-induced chemotaxis of hepatic stellate cells in liver fibrosis
Gong J, Han J, He J, et al. Lab Invest, 97(9): 1020-1032.
Gastric electrical stimulation improves enteric neuronal survival
Wang, Nian, et al. International Journal of Molecular Medicine 40.2 (2017): 438-446.
Rifaximin exerts beneficial effects in PI-IBS mouse model beyond gut microbiota
Jin, Yu, et al. Journal of Gastroenterology and Hepatology (2017).
Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
Liu, Yuan, et al. BMC infectious diseases 17.1 (2017): 403.
Melatonin promoted renal regeneration in folic acid-induced acute kidney injury via inhibiting nucleocytoplasmic translocation of HMGB1 in tubular epithelial cells
Zhu, Fengming, et al. American journal of translational research 9.4 (2017): 1694.
P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis
Niu, Xuan, et al. Arteriosclerosis, Thrombosis, and Vascular Biology (2017): ATVBAHA-116.
Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs in vitro
Chen, Xu, et al. PloS one 12.2 (2017): e0172744.
Molecular cloning, characterization and tissue specificity of the expression of the ovine CSRP2 and CSRP3 genes from Small-tail Han sheep (Ovis aries)
Liu, G, et al. Gene 580.1(2016): 47-57.
Ikaros 6 protects acute lymphoblastic leukemia cells against daunorubicin-induced apoptosis by activating the Akt-FoxO1 pathway
Han, Juan, et al. Journal of Leukocyte Biology (2016): jlb-2A0116.
Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury.
Zuo Y, Xiong N, Zhao H. Journal of Huazhong University of Science and Technology [Medical Sciences], 2016, 36(4): 548-553.
Effects of Buscopan on human gastrointestinal smooth muscle activity in an ex vivo model: Are there any differences for various sections.
Zhang L, Song J, Bai T, et al. European journal of pharmacology, 2016, 780: 180-187.
Establishment and characterization of a novel osteosarcoma cell line: CHOS.
Liu Y, Feng X, Zhang Y, et al. Journal of Orthopaedic Research, 2016.
Blocking Nuclear Factor-Kappa B Protects against Diet-Induced Hepatic Steatosis and Insulin Resistance in Mice.
Zeng T, Zhou J, He L, et al. PloS one, 2016, 11(3): e0149677.
Possible mechanism by which renal sympathetic denervation improves left ventricular remodelling after myocardial infarction.
Zheng X X, Li X Y, Lyu Y N, et al. Experimental physiology, 2016, 101(2): 260-271.
Renal Sympathetic Denervation in Rats Ameliorates Cardiac Dysfunction and Fibrosis Post-Myocardial Infarction Involving MicroRNAs.
Zheng X, Li X, Lyu Y, et al. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 2016, 22: 2751.
Metabolic factors-triggered inflammatory response drives antidepressant effects of exercise in CUMS rats.
Liu W, Wang H, Wang Y, et al. Psychiatry research, 2015, 228(3): 257-264.
Depletion of Kupffer cells attenuates systemic insulin resistance, inflammation and improves liver autophagy in high-fat diet fed mice.
Zeng T, Liu F, Zhou J, et al. Endocrine journal, 2015 (0).
Downregulation of Sprouty homolog 2 by microRNA-21 inhibits proliferation, metastasis and invasion, however promotes the apoptosis of multiple myeloma cells.
Wang J H, Zhou W W, Cheng S T, et al. Molecular medicine reports, 2015, 12(2): 1810-1816.
Increased expression of formin-like 3 contributes to metastasis and poor prognosis in colorectal carcinoma.
Zeng Y F, Xiao Y S, Lu M Z, et al. Experimental and molecular pathology, 2015, 98(2): 260-267.
Correlation between microRNA‑21 and sprouty homolog 2 gene expression in multiple myeloma.
Wang J H, Zheng W W, Cheng S T, et al. Molecular medicine reports, 2015, 11(6): 4220-4224.