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Anti-GAPDH Mouse Monoclonal Antibody (2B5)

Product Information

  • Product name

    Anti-GAPDH Mouse Monoclonal Antibody (2B5)

  • Immunogen

    Synthetic Peptide

  • Host


  • Reactivity

    Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast

  • Applications

    IF, IHC-P, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400).

  • Clonality


  • Isotype

    Mouse IgG1

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.5. Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.7 Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

Product Properties

  • Formulation

    Liquid solution

  • Concentration

    1 mg/ml

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.

  • Gene ID


  • Alternative names

    GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

  • Accession


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

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  3. Acupuncture Reduces Apoptosis of Granulosa Cells in Rats with Premature Ovarian Failure Via Restoring the PI3K/Akt Signaling Pathway

    S Wang, S Lin, M Zhu. International Journal of Molecular Sciences

  4. Rod bipolar cells dysfunction occurs before ganglion cells loss in excitotoxin-damaged mouse retina

    Y Shen, X Luo. Cell Death & Disease

  5. PITPNA-AS1 abrogates the inhibition of miR-876-5p on WNT5A to facilitate hepatocellular carcinoma progression

    J Sun, Y Zhang, B Li. Cell Death & Disease

  6. All‑trans‑retinoic acid modulates TGF‑β‑induced apoptosis, proliferation, migration and extracellular matrix synthesis of conjunctival fibroblasts by inhibiting PI3K/AKT signaling

    L Liang, X Wang, Y Zheng. Molecular medicine Reports, 2019.

  7. Electroacupuncture at ST-36 ameliorates DSS-induced acute colitis via regulating macrophage polarization induced by suppressing NLRP3/IL-1β and promoting Nrf2.

    S Song, J An, Y Li, S Liu - Molecular immunology, 2019.

  8. L-Fucose ameliorates DSS-induced acute colitis via inhibiting macrophage M1 polarization and inhibiting NLRP3 inflammasome and NF-kB activation

    R He, Y Li, C Han, R Lin, W Qian, X Hou. International Immunopharmacology, 2019.

  9. Alleviation of Mechanical Allodynia by 14,15-Epoxyeicosatrienoic Acid in a Central Poststroke Pain Model: Possible Role of Allopregnanolone and δ-Subunit-Containing Gamma-Aminobutyric Acid A Receptors

    X Chen, Z Li, B Zhang, R Hu, J Li, M Feng, W Yao. The Journal of pain, 2019.

  10. Autism-like social deficit generated by Dock4 deficiency is rescued by restoration of Rac1 activity and NMDA receptor function

    D Guo, Y Peng, L Wang, X Sun, X Wang, C Liang. Molecular Psychiatry, 2019.

  11. Alleviation of Mechanical Allodynia by 14,15-Epoxyeicosatrienoic Acid in a Central Poststroke Pain Model: Possible Role of Allopregnanolone and δ-Subunit-Containing Gamma-Aminobutyric Acid A Receptors

    Xuhui Chen, Zuofan Li, BoZhang, et al. J Pain. 2019 May;20(5):577-591.

  12. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis

    Shanshan Nie, Yuhang Zhao, Xinjian Qiu, et al. Evidence-Based Complementary and Alternative Medicine.

  13. L-Fucose ameliorates DSS-induced acute colitis via inhibiting macrophage M1 polarization and inhibiting NLRP3 inflammasome and NF-kB activation

    Ruohang He, Ying Li, Chaoqun Han, et al. Int Immunopharmacol. 2019 May 24;73:379-388.

  14. NLRP3 inflammasome and lipid metabolism analysis based on UPLC-Q-TOF-MS in gouty nephropathy

    Yan‑Zi Zhang, Xiao‑Lu Sui, Yun‑Peng Xu, et al. Int J Mol Med. 2019 Jul;44(1):172-184.

  15. Bisphenol S promotes the cell cycle progression and cell proliferation through ERα-cyclin D-CDK4/6-pRb pathway in MCF-7 breast cancer cells

    Zhenxian Lin, Xiaona Zhang, Fei Zhao and Shaoguo Ru. Toxicol Appl Pharmacol. 2019 Mar 1;366:75-82.

  16. Compound α-keto acid tablet supplementation alleviates chronic kidney disease progression via inhibition of the NF-kB and MAPK pathways

    Meng Wang, Huzi Xu, Octavia Li‑Sien Chong Lee Shin, et al. J Transl Med. 2019 Apr 11;17(1):122.

  17. Bone marrow-derived Ly6C− macrophages promote ischemia-induced chronic kidney disease

    Qian Yang, Yuxi Wang, Guangchang Pei, et al. Cell Death Dis. 2019 Mar 29;10(4):291.

  18. Tumoral microvesicle–activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma

    Erhui Jiang, Zhi Xu, Meng Wang, et al. FASEB J. 2019 Apr;33(4):5690-5703.

  19. Gut inflammation exacerbates hepatic injury in the high-fat diet induced NAFLD mouse: Attention to the gut-vascular barrier dysfunction

    Chunwei Cheng, Jun Tan, et al. Life Sciences. 2018; 209: 157-166.

  20. Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase(TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice

    Yuan Liu, Aiping Cao, et al. BMC Infectious Diseases. 2017; 17(1): 403.

  21. Beneficial effects of Rifaximin in post‐infectious irritable bowel syndrome mouse model beyond gut microbiota

    Yu Jin, Xiaoyang Ren, et al. Journal of Gastroenterology and Hepatology. 2018; 33(2): 443-452.

  22. Hydrogen attenuated oxidized low‑density lipoprotein‑induced inflammation through the stimulation of autophagy via sirtuin 1

    Sen Yang, Ju He, et al. Experimental and Therapeutic Medicine. 2018; 16(5): 4042-4048.

  23. GAG and collagen II attenuate glucocorticoid-induced osteoporosis by regulating NF-κB and MAPK signaling

    Han Zhu, Meng Wang, et al. American Journal of Translational Research. 2018 ; 10(6): 1762-1772.

  24. Melatonin promoted renal regeneration in folic acid-induced acute kidney injury via inhibiting nucleocytoplasmic translocation of HMGB1 in tubular epithelial cells

    Fengming Zhu, Ying Yao, et al. American Journal of Translational Research. 2017; 9(4): 1694-1707.

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    Wang S, Song Y, Wang Y, et al. Chemical Research in Chinese Universities, 2018, 34(1): 67–74.

  29. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria

    Liu H, Liu Y, Li Y, et al. Exp Ther Med, 2018, 15(3): 2525-2532.

  30. Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro

    Ren L, Wang P, Wang Z, et al. Mol Med Rep,16(4): 4334-4340.

  31. Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2

    Zhang L, Song J, Bai T, et al. Sci Rep, 2017, 10; 7(1): 4950.

  32. Paired related homeobox protein 1 regulates PDGF-induced chemotaxis of hepatic stellate cells in liver fibrosis

    Gong J, Han J, He J, et al. Lab Invest, 97(9): 1020-1032.

  33. P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis

    Niu, Xuan, et al. Arteriosclerosis, Thrombosis, and Vascular Biology (2017): ATVBAHA-116.

  34. Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs in vitro

    Chen, Xu, et al. PloS one 12.2 (2017): e0172744.

  35. Molecular cloning, characterization and tissue specificity of the expression of the ovine CSRP2 and CSRP3 genes from Small-tail Han sheep (Ovis aries)

    Liu, G, et al. Gene 580.1(2016): 47-57.

  36. Ikaros 6 protects acute lymphoblastic leukemia cells against daunorubicin-induced apoptosis by activating the Akt-FoxO1 pathway

    Han, Juan, et al. Journal of Leukocyte Biology (2016): jlb-2A0116.

  37. Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury

    Zuo Y, Xiong N, Zhao H. Journal of Huazhong University of Science and Technology [Medical Sciences], 2016, 36(4): 548-553.

  38. Effects of Buscopan on human gastrointestinal smooth muscle activity in an ex vivo model: Are there any differences for various sections

    Zhang L, Song J, Bai T, et al. European journal of pharmacology, 2016, 780: 180-187.

  39. Establishment and characterization of a novel osteosarcoma cell line: CHOS

    Liu Y, Feng X, Zhang Y, et al. Journal of Orthopaedic Research, 2016.

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    Zeng T, Zhou J, He L, et al. PloS one, 2016, 11(3): e0149677.

  41. Possible mechanism by which renal sympathetic denervation improves left ventricular remodelling after myocardial infarction

    Zheng X X, Li X Y, Lyu Y N, et al. Experimental physiology, 2016, 101(2): 260-271.

  42. Metabolic factors-triggered inflammatory response drives antidepressant effects of exercise in CUMS rats

    Liu W, Wang H, Wang Y, et al. Psychiatry research, 2015, 228(3): 257-264.

  43. Depletion of Kupffer cells attenuates systemic insulin resistance, inflammation and improves liver autophagy in high-fat diet fed mice

    Zeng T, Liu F, Zhou J, et al. Endocrine journal, 2015 (0).

  44. Downregulation of Sprouty homolog 2 by microRNA-21 inhibits proliferation, metastasis and invasion, however promotes the apoptosis of multiple myeloma cells

    Wang J H, Zhou W W, Cheng S T, et al. Molecular medicine reports, 2015, 12(2): 1810-1816.

  45. Increased expression of formin-like 3 contributes to metastasis and poor prognosis in colorectal carcinoma

    Zeng Y F, Xiao Y S, Lu M Z, et al. Experimental and molecular pathology, 2015, 98(2): 260-267.

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  47. Renal Sympathetic Denervation in Rats Ameliorates Cardiac Dysfunction and Fibrosis Post-Myocardial Infarction Involving MicroRNAs

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Cat #: A01020

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

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