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Anti-E2 Tag Mouse Monoclonal Antibody (12T4)

Anti-E2 Tag Mouse Monoclonal Antibody (12T4)

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Specification

Product name Anti-E2 Tag Mouse Monoclonal Antibody (12T4)
Immunogen Synthetic Peptide
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative E2 tag; E2 epitope; SSTSSDFRDR epitope; SSTSSDFRDR tag

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The E2 Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids (SSTSSDFRDR). The detection of E2 tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein. E2 Tag antibody can recognize C-terminal, internal, and N-terminal E2 tagged proteins.
Alternative E2 tag; E2 epitope; SSTSSDFRDR epitope; SSTSSDFRDR tag

Image & description

Fig. Western blot analysis of 2ug E2 fusion protein with Anti-E2 monoclonal antibody in 1:2000 (lane A) and 1:5000 (lane B) dilutions.

Fig. Western blot analysis of 2ug E2 fusion protein with Anti-E2 monoclonal antibody in 1:2000 (lane A) and 1:5000 (lane B) dilutions.

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