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Anti-DDDDK Tag Rabbit Polyclonal Antibody

Anti-DDDDK Tag Rabbit Polyclonal Antibody

Views(133) Publications(1) Catalog no(A02011)
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Specification

Product name Anti-DDDDK Tag Rabbit Polyclonal Antibody
Immunogen Synthetic Peptide
Host Rabbit
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Alternative DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope

Image & description

Fig. Western blot analysis of 1ug Flag fusion protein with Anti-Flag rabbit polyclonal antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.

Fig. Western blot analysis of 1ug Flag fusion protein with Anti-Flag rabbit polyclonal antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.

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