Product Information

• Product name

  Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)

• Immunogen

  Synthetic Peptide

• Host

  Mouse

• Applications

  IF, IP, WB

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).

• Clonality

  Monoclonal

• Isotype

  Mouse IgG

• Purification

  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.

Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).

Product Properties

• Formulation

  Liquid solution

• Concentration

  1 mg/ml

• Storage buffer

  Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

• Alternative names

  DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope