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Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)

Product Information

  • Product name

    Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)

  • Immunogen

    Synthetic Peptide

  • Host


  • Applications

    IF, IP, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).

  • Clonality


  • Isotype

    Mouse IgG

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.

Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).

Product Properties

  • Formulation

    Liquid solution

  • Concentration

    1 mg/ml

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

  • Alternative names

    DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope


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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Ameliorative effect of docosahexaenoic acid on hepatocyte apoptosis and inflammation induced by oleic acid in grass carp, Ctenopharyngodon idella

    A Jin, X Shi, W Deng, J Sun, H Ji. Fish physiology and biochemistry, 2019.

  2. Molecular cloning and functional characterization of porcine 2′,5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome virus

    R Wang, Y Kang, H Li, H Ma, W Wang, Y Cheng. Veterinary Immunology and Immunopathology, 2019.

  3. Retardation of the Calvin cycle contributes to the reduced CO2 assimilation ability of rice stripe virus-infected N. benthamiana and suppresses viral infection

    J Bi, Y Yang, B Chen, J Zhao, Z Chen, B Song. Front. Microbiol., 20 March 2019.

  4. An RXLR effector secreted by Phytophthora parasitica is a virulence factor and triggers cell death in various plants

    G Huang, Z Liu, B Gu, H Zhao, J Jia. Molecular plant pathology,2019.

  5. Epicatechin Gallate Protects HBMVECs from Ischemia/Reperfusion Injury through Ameliorating Apoptosis and Autophagy and Promoting Neovascularization

    B Fu, Q Zeng, Z Zhang, M Qian, J Chen. Oxidative Medicine and Cellular Longevity, Volume 2019, Article ID 7824684, 11 pages.

  6. Non-stick hemostasis hydrogels as dressings with bacterial barrier activity for cutaneous wound healing

    H Pan, D Fan, Z Duan, C Zhu, R Fu, X Li. Materials Science and Engineering: C, Volume 105, December 2019.

  7. Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins

    Ting Ouyang, Guyu Niu, Yifang Zhang, et al. Viruses. 2019 Jun 11;11(6).

  8. Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer׳s disease

    Qian Guo, Xiaoyao Zheng, Peng Yang, et al. Acta Pharm Sin B. 2019 May;9(3):590-603.

  9. DNA methylation and hydroxymethylation are associated with the degree of coronary atherosclerosis in elderly patients with coronary heart disease

    Dan Jiang, Min Sun, Linna You, et al. Life Sci. 2019 May 1;224:241-248.

  10. TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis

    Jinyuan Cui, Jia Hu, Zhilan Ye, et al. Science Bulletin Available online 25 May 2019

  11. Molecular cloning and functional characterization of porcine 2′,5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome viru

    Ruining Wang, Yinfeng Kang, Huawei Li, et al. Vet Immunol Immunopathol. 2019 Mar;209:22-30.

  12. Ameliorative effect of docosahexaenoic acid on hepatocyte apoptosis and inflammation induced by oleic acid in grass carp, Ctenopharyngodon idella

    Ai Jin, Xiao-chen Shi, Wei Deng, et al. Fish Physiol Biochem. 2019 Mar 21.

  13. Retardation of the Calvin Cycle Contributes to the Reduced CO2 Assimilation Ability of Rice Stripe Virus-Infected N. benthamiana and Suppresses Viral Infection

    Ji’an Bi, Yong Yang, Binghua Chen, et al. Front Microbiol. 2019 Mar 20;10:568.

  14. Protein interactome of the deamidase phosphoribosylformylglycinamidine synthetase (PFAS) by LC-MS/MS

    Ai Lu, Cyrollah Disoma,YuzhengZhou, et al. Biochemical and Biophysical Research Communications, 13 April 2019

  15. Molecular Cloning and Identification of the 2′–5′ Oligoadenylate Synthetase 2 Gene in Chinese Domestic Pigs Through Bioinformatics Analysis, and Determination of Its Antiviral Activity Against Porcine Reproductive and Respiratory Syndrome Virus Infection

    Ruining Wang, Hongfang Ma, et al. Indian Journal of Microbiology. 2018; 58(3): 332-344.

  16. Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release

    Jigang Wang, Ti Fang, et al. Journal of Materials Chemistry B. 2018; 45: 7345-7558.

  17. An RXLR effector secreted by Phytophthora parasitica is a virulence factor and triggers cell death in various plants

    Guiyan Huang, Zhirou Liu, et al. Molecular Plant Pathology. (2018)

  18. Formin-like 3 regulates RhoC/FAK pathway and actin assembly to promote cell invasion in colorectal carcinoma

    Yuan-Feng Zeng, Yi-Sheng Xiao, et al. World Journal of Gastroenterology. 2018; 24(34): 3884-3897.

  19. Dietary docosahexaenoic acid decreased lipid accumulation via inducing adipocytes apoptosis of grass carp, Ctenopharygodon idella

    Jin A, Lei CX, Tian JJ, et al. Fish Physiol Biochem, 2018, 44(1): 197-207.

  20. FBW7 loss promotes epithelial-to-mesenchymal transition in non-small cell lung cancer through the stabilization of Snail protein

    Zhang Y, Zhang X X, Ye M X, et al. Cancer letters, 2018, 419: 75-83.

  21. Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs in vitro

    Chen, Xu, et al. PloS one 12.2 (2017): e0172744.

  22. Different Phenotypes of the Two Chinese Probands with the Same c.889G>A (p.C162Y) Mutation in COCH Gene Verify Different Mechanisms Underlying Autosomal Dominant Nonsyndromic Deafness 9

    Wang, Qi, et al. PloS one 12.1 (2017): e0170011.

  23. GATA binding protein 3 is correlated with leptin regulation of PPARγ1 in hepatic stellate cells

    Guan, Wei, et al. Journal of Cellular and Molecular Medicine (2016).

  24. Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis

    Lv, Meinan, et al. Journal of the American Chemical Society 138.20 (2016): 6427-6435.

  25. Interferon‐inducible Protein 6‐16 (IFI‐6‐16, ISG16) promotes Hepatitis C virus replication in vitro.

    Chen S, Li S, Chen L. Journal of medical virology, 2016, 88(1): 109-114.

Cat #: A02010

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

Contact information

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