- Product name
Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)
IF, IP, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.
Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
- Alternative names
DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope
Most popular with customers
Application: IF, IP, WB
Application: IF, IP, WB
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Molecular cloning and functional characterization of porcine 2′,5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome viru
Ruining Wang, Yinfeng Kang, Huawei Li, et al. Vet Immunol Immunopathol. 2019 Mar;209:22-30.
Ameliorative effect of docosahexaenoic acid on hepatocyte apoptosis and inflammation induced by oleic acid in grass carp, Ctenopharyngodon idella
Ai Jin, Xiao-chen Shi, Wei Deng, et al. Fish Physiol Biochem. 2019 Mar 21.
Retardation of the Calvin Cycle Contributes to the Reduced CO2 Assimilation Ability of Rice Stripe Virus-Infected N. benthamiana and Suppresses Viral Infection
Ji’an Bi, Yong Yang, Binghua Chen, et al. Front Microbiol. 2019 Mar 20;10:568.
Protein interactome of the deamidase phosphoribosylformylglycinamidine synthetase (PFAS) by LC-MS/MS
Ai Lu, Cyrollah Disoma,YuzhengZhou, et al. Biochemical and Biophysical Research Communications, 13 April 2019
Molecular Cloning and Identification of the 2′–5′ Oligoadenylate Synthetase 2 Gene in Chinese Domestic Pigs Through Bioinformatics Analysis, and Determination of Its Antiviral Activity Against Porcine Reproductive and Respiratory Syndrome Virus Infection
Ruining Wang, Hongfang Ma, et al. Indian Journal of Microbiology. 2018; 58(3): 332-344.
Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release
Jigang Wang, Ti Fang, et al. Journal of Materials Chemistry B. 2018; 45: 7345-7558.
An RXLR effector secreted by Phytophthora parasitica is a virulence factor and triggers cell death in various plants
Guiyan Huang, Zhirou Liu, et al. Molecular Plant Pathology. (2018)
Formin-like 3 regulates RhoC/FAK pathway and actin assembly to promote cell invasion in colorectal carcinoma
Yuan-Feng Zeng, Yi-Sheng Xiao, et al. World Journal of Gastroenterology. 2018; 24(34): 3884-3897.
Dietary docosahexaenoic acid decreased lipid accumulation via inducing adipocytes apoptosis of grass carp, Ctenopharygodon idella
Jin A, Lei CX, Tian JJ, et al. Fish Physiol Biochem, 2018, 44(1): 197-207.
FBW7 loss promotes epithelial-to-mesenchymal transition in non-small cell lung cancer through the stabilization of Snail protein
Zhang Y, Zhang X X, Ye M X, et al. Cancer letters, 2018, 419: 75-83.
Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs in vitro
Chen, Xu, et al. PloS one 12.2 (2017): e0172744.
Different Phenotypes of the Two Chinese Probands with the Same c.889G>A (p.C162Y) Mutation in COCH Gene Verify Different Mechanisms Underlying Autosomal Dominant Nonsyndromic Deafness 9
Wang, Qi, et al. PloS one 12.1 (2017): e0170011.
GATA binding protein 3 is correlated with leptin regulation of PPARγ1 in hepatic stellate cells
Guan, Wei, et al. Journal of Cellular and Molecular Medicine (2016).
Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis
Lv, Meinan, et al. Journal of the American Chemical Society 138.20 (2016): 6427-6435.
Interferon‐inducible Protein 6‐16 (IFI‐6‐16, ISG16) promotes Hepatitis C virus replication in vitro.
Chen S, Li S, Chen L. Journal of medical virology, 2016, 88(1): 109-114.