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Anti-beta Actin Rabbit Polyclonal Antibody

Anti-beta Actin Rabbit Polyclonal Antibody

Views(129) Publications(4) Catalog no(A01011)
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Specification

Product name Anti-beta Actin Rabbit Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Chicken, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xneous
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:3000), IHC-P (1:200).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative ACTB; Actin, cytoplasmic 1; Beta-actin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ACTB encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Gene ID 60
Alternative ACTB; Actin, cytoplasmic 1; Beta-actin
Others The antibody detects endogenous β-actin protein.
Accession P60709
Observed Band(KD) 43

Image & description

Fig.1. Western blot analysis of HepG2 (1), Rat liver (2), Mouse kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000. Secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of HepG2 (1), Rat liver (2), Mouse kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000. Secondary antibody was diluted at 1:20000.

Fig.2. IHC-P staining of Human ovary tissue, diluted at 1:200.

Fig.2. IHC-P staining of Human ovary tissue, diluted at 1:200.

This product has been cited in4publications

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