- Product name
Anti-β-Tubulin Mouse Monoclonal Antibody (3G6)
Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast
IF, IHC-p, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400).
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Fig.1. Western blot analysis of A549(1), rat brain (2), mouse brain (3), chicken lung (4) and rabbit testis(5), sheep muscle(6), diluted at 1:10000.
Fig.2. Immunofluorescence analysis of human appendix tissue. 1, β-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunofluorescence analysis of mouse lung tissue. 1, β-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, β-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
Fig.5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, β-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kDa. Antibodies against β-Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells, such as adipose tissue.
- Gene ID
- Alternative names
TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Most popular with customers
Application: IF, IHC-p, WB
Reactivity: Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
Application: IF, IHC-p, WB
Reactivity: Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat, Sheep, Yeast
Application: IF, IHC-p, WB
Reactivity: Human, Mouse, Rat
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Using CETSA assay and a mathematical model to reveal dual Bcl-2/Mcl-1 inhibition and on-target mechanism for ABT-199 and S1
Z Guo, T Song, Z Xue. European Journal of Pharmaceutical Sciences
Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model
S Wang, N Wang, B Yu, M Cao, Y Wang, Y Guo. Oncogene volume 38, pages6338–6353 (2019).
MiR-34b-5p Mediates the Proliferation and Differentiation of Myoblasts by Targeting IGFBP2
Zhijun Wang, Xiaocui Zhang, Zhenhui Li, et al. Cells. 2019 Apr 17;8(4). pii: E360.
Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic β Cell Identity in db/db Mice
Fen Yuan, Dingkun Wang, Leyi Ma, et al. Evidence-Based Complementary and Alternative Medicine Volume 2019, Article ID 1232913, 13 pages
Tumoral microvesicle–activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma
Erhui Jiang, Zhi Xu, Meng Wang, et al. FASEB J. 2019 Apr;33(4):5690-5703.
Expression of WNT1 in ameloblastoma and its significance
Guan‑Nan Wang, Ming Zhong, et al. Oncology Letters. 2018; 16(2): 1507-1512.
Construction of the IRES-based Vector for Multiple Gene Co-expression
Tian C H, Xie X M, Li Y, et al. China Biotechnology , 2017, 37(7): 97-106.
Lost region in amyloid precursor protein (APP) through TALEN-mediated genome editing alters mitochondrial morphology.
Wang Y, Wu F, Pan H, et al. Scientific reports, 2016, 6.