- Product name
Anti-β-Actin Mouse Monoclonal Antibody (1C7)
Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
IF, IHC-p, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IHC-P (1:200), IF (1:50-1:200).
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
- Storage buffer
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.
- Gene ID
- Alternative names
ACTB; Actin; cytoplasmic 1; Beta-actin
Most popular with customers
Application: IF, IHC, IP, WB
Reactivity: Human, Mouse, Rat, Yeast
Application: IF, IHC-p, WB
Reactivity: Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
Application: IF, IHC, IP, WB
Reactivity: Human, Mouse, Rat
Application: IF, IHC, WB
Reactivity: Human, Mouse, Rat
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
Roles of cellular NSF protein in entry and nuclear egress of budded virions of Autographa californica multiple nucleopolyhedrovirus
Guo Y, Yue Q, Gao J, et al. J Virol, 2017, 91(20).
Sema4D/PlexinB1 inhibition ameliorates blood-brain barrier damage and improves outcome after stroke in rats
Zhou YF, Li YN, Jin HJ, et al. FASEB J, 2018, 32(4): 2181-2196.
Effects of hyperbaric factors on lidocaine-induced apoptosis in spinal neurons and the role of p38 mitogen-activated protein kinase in rats with diabetic neuropathic pain
Zheng X, Chen L, Du X, et al. Exp Ther Med, 2017, 13(6): 2855-2861.
The critical role of p16/Rb pathway in the inhibition of GH3 cell cycle induced by T-2 toxin
Fatima Z, Guo P, Huang D, et al. Toxicology, 2018, 19, 400-401: 28-39.
Over-expression of Oryza sativa Xrn4 confers plant resistance to virus infection
Jiang S, Jiang L, Yang J, et al. Gene, 2018, 10; 639: 44-51.
Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells
Liu Y, Fu Q Z, Pu L, et al. Mol Med Rep, 2017, 16(4): 5107-5112.
Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression
Ji S Y, Liu M, Zhang Y P, et al. BioMed Research International, 2018.
Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2
Zhang L, Song J, Bai T, et al. Sci Rep, 2017, 10; 7(1): 4950.
Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats
Liu Q Q, Liu H, He Z G, et al. Oncotarget, 2017, 8(32): 53465–53481.
The dual role of poly(ADP-ribose) polymerase-1 in modulating parthanatos and autophagy under oxidative stress in rat cochlear marginal cells of the stria vascularis
Jiang H Y, Yang Y, Zhang Y Y, et al. Redox Biology, 2018, 14: 361-370.
Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells
Yan, Yun, et al. Scientific Reports 7 (2017).
Establishment of insect cell lines expressing green fluorescent protein on cell surface based on AcMNPV GP64 membrane fusion characteristic
Qi, Ben-Xiang, et al. Cytotechnology (2017): 1-9.
Ginsenoside Rg1 attenuates the inflammatory response in DSS-induced mice colitis
Zhu, Guo, et al. International Immunopharmacology 50 (2017): 1-5.
P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis
Niu, Xuan, et al. Arteriosclerosis, Thrombosis, and Vascular Biology (2017): ATVBAHA-116.
Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs in vitro
Chen, Xu, et al. PloS one 12.2 (2017): e0172744.
ISG12a inhibits HCV replication and potentiates the anti-HCV activity of IFN-α through activation of the Jak/STAT signaling pathway independent of autophagy and apoptosis
Chen, Y, et al. Virus Research 227(2017):231-239
Identification and functional characterization of a novel thyrotropin receptor mutation (V87L) in a Chinese woman with subclinical hypothyroidism
Zhang, HM, et al. Experimental and Therapeutic Medicine 131 (2017): 290-294
A Novel Mechanism of Action for Salidroside to Alleviate Diabetic Albuminuria: Effects on Albumin Transcytosis across Glomerular Endothelial Cells
Wu, D, et al. American Journal of Physiology-Endocrinology and Metabolism, 2016, 310(3): E225-E237.
Expression of Smad1 in renal tubular epithelial cells in high glucose condition
Chen Meilian, et al. China Tropical Medicine, 15.1 (2015): 8-10.
Inflammasome-independent role of NLRP12 in suppressing colonic inflammation regulated by Blimp-1.
Shi F, Yang Y, Kouadir M, et al. Oncotarget, 2016.
MicroRNA-150 regulates blood–brain barrier permeability via Tie-2 after permanent middle cerebral artery occlusion in rats.
Fang Z, He Q W, Li Q, et al. The FASEB Journal, 2016, 30(6): 2097-2107.