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Anti-β-Actin Mouse Monoclonal Antibody (1C7)

Anti-β-Actin Mouse Monoclonal Antibody (1C7)

Views(403) Publications(42) Catalog no(A01010)
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Specification

Product name Anti-β-Actin Mouse Monoclonal Antibody (1C7)
Immunogen Synthetic Peptide
Host Mouse
Reactivity Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat
Applications IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:100-1:400).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative ACTB; Actin; cytoplasmic 1; Beta-actin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.
Gene ID 60
Alternative ACTB; Actin; cytoplasmic 1; Beta-actin
Accession P60709
Observed Band(KD) 43

Image & description

Fig.1.Western blot analysis (1:10,000) of β-Actin expression in Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3), Chicken lung (lane 4) and Rabbit testis (lane 5) with Anti-β-Actin mouse monoclonal antibody (1C7).

Fig.1.Western blot analysis (1:10,000) of β-Actin expression in Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3), Chicken lung (lane 4) and Rabbit testis (lane 5) with Anti-β-Actin mouse monoclonal antibody (1C7).

Fig.2. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

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