Anti-β-Actin Mouse Monoclonal Antibody (1C7)

Product Information

  • Product name

    Anti-β-Actin Mouse Monoclonal Antibody (1C7)

  • Immunogen

    Synthetic Peptide

  • Host

    Mouse

  • Reactivity

    Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, Rat

  • Applications

    IF, IHC-p, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:100-1:400).

  • Clonality

    Monoclonal

  • Isotype

    Mouse IgG1

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Western blot analysis of hela (1), rat brain (2), Mouse brain (3), chicken lung (4) and rabbit testis (5), diluted at 1:10000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, β-actin Monoclonal Antibody (1C7) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Product Properties

  • Formulation

    Liquid solution

  • Concentration

    1 mg/ml

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.

  • Gene ID

    60

  • Alternative names

    ACTB; Actin; cytoplasmic 1; Beta-actin

  • Accession

    P60709

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

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    Ai Lu, Cyrollah Disoma,YuzhengZhou, et al. Biochemical and Biophysical Research Communications, 13 April 2019

  9. Low density lipoprotein mimics insulin action on autophagy and glucose uptake in endothelial cells

    Lin Zhu, Guangjie Wu, Xiaoyan Yang, et al. Sci Rep. 2019 Feb 28;9(1):3020.

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  11. Seipin deletion in mice enhances phosphorylation and aggregation of tau protein through reduced neuronal PPARγ and insulin resistance

    Huanxian Chang, TingtingDi, YaWang, et al. Neurobiol Dis. 2019 Mar 22;127:350-361.

  12. Tumoral microvesicle–activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma

    Erhui Jiang, Zhi Xu, Meng Wang, et al. FASEB J. 2019 Apr;33(4):5690-5703.

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    Xiao dong Zhang, Yan Li, et al. Journal of Experimental & Clinical Cancer Research. 2018; 37(1): 216.

  15. Roles of cellular NSF protein in entry and nuclear egress of budded virions of Autographa californica multiple nucleopolyhedrovirus

    Guo Y, Yue Q, Gao J, et al. J Virol, 2017, 91(20).

  16. Sema4D/PlexinB1 inhibition ameliorates blood-brain barrier damage and improves outcome after stroke in rats

    Zhou YF, Li YN, Jin HJ, et al. FASEB J, 2018, 32(4): 2181-2196.

  17. Effects of hyperbaric factors on lidocaine-induced apoptosis in spinal neurons and the role of p38 mitogen-activated protein kinase in rats with diabetic neuropathic pain

    Zheng X, Chen L, Du X, et al. Exp Ther Med, 2017, 13(6): 2855-2861.

  18. The critical role of p16/Rb pathway in the inhibition of GH3 cell cycle induced by T-2 toxin

    Fatima Z, Guo P, Huang D, et al. Toxicology, 2018, 19, 400-401: 28-39.

  19. Over-expression of Oryza sativa Xrn4 confers plant resistance to virus infection

    Jiang S, Jiang L, Yang J, et al. Gene, 2018, 10; 639: 44-51.

  20. Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells

    Liu Y, Fu Q Z, Pu L, et al. Mol Med Rep, 2017, 16(4): 5107-5112.

  21. Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression

    Ji S Y, Liu M, Zhang Y P, et al. BioMed Research International, 2018.

  22. Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2

    Zhang L, Song J, Bai T, et al. Sci Rep, 2017, 10; 7(1): 4950.

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    Qi, Ben-Xiang, et al. Cytotechnology (2017): 1-9.

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    Zhu, Guo, et al. International Immunopharmacology 50 (2017): 1-5.

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    Niu, Xuan, et al. Arteriosclerosis, Thrombosis, and Vascular Biology (2017): ATVBAHA-116.

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    Chen, Xu, et al. PloS one 12.2 (2017): e0172744.

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    Chen, Y, et al. Virus Research 227(2017):231-239

  31. Identification and functional characterization of a novel thyrotropin receptor mutation (V87L) in a Chinese woman with subclinical hypothyroidism

    Zhang, HM, et al. Experimental and Therapeutic Medicine 131 (2017): 290-294

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    Wu, D, et al. American Journal of Physiology-Endocrinology and Metabolism, 2016, 310(3): E225-E237.

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    Chen Meilian, et al. China Tropical Medicine, 15.1 (2015): 8-10.

  34. Inflammasome-independent role of NLRP12 in suppressing colonic inflammation regulated by Blimp-1.

    Shi F, Yang Y, Kouadir M, et al. Oncotarget, 2016.

  35. MicroRNA-150 regulates blood–brain barrier permeability via Tie-2 after permanent middle cerebral artery occlusion in rats.

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Cat #: A01010

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860

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