Anti-β-Actin Mouse Monoclonal Antibody (1C7)

Product Information

  • Product name

    Anti-β-Actin Mouse Monoclonal Antibody (1C7)

  • Immunogen

    Synthetic Peptide

  • Host

    Mouse

  • Reactivity

    Chicken, Dog, Human, Insect, Monkey, Mouse, Rabbit, Rat

  • Applications

    IHC, WB

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:5000, IHC-p 1:200.

  • Clonality

    Monoclonal

  • Isotype

    Mouse IgG1

  • Purification

    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Fig.1.Immunohistochemistry staining (1:200) of β-Actin in Human ovary tissue.

Fig.2.Western blot analysis (1:10,000) of β-Actin expression in Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3), Chicken lung (lane 4) and Rabbit testis (lane 5) with Anti-β-Actin mouse monoclonal antibody (1C7).

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.

  • Gene ID

    60

  • Alternative names

    ACTB; Actin; cytoplasmic 1; Beta-actin

  • Accession

    P60709

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

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  2. Establishment of insect cell lines expressing green fluorescent protein on cell surface based on AcMNPV GP64 membrane fusion characteristic

    Qi, Ben-Xiang, et al. Cytotechnology (2017): 1-9.

  3. Ginsenoside Rg1 attenuates the inflammatory response in DSS-induced mice colitis

    Zhu, Guo, et al. International Immunopharmacology 50 (2017): 1-5.

  4. P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis

    Niu, Xuan, et al. Arteriosclerosis, Thrombosis, and Vascular Biology (2017): ATVBAHA-116.

  5. Severe fever with thrombocytopenia syndrome virus inhibits exogenous Type I IFN signaling pathway through its NSs in vitro

    Chen, Xu, et al. PloS one 12.2 (2017): e0172744.

  6. ISG12a inhibits HCV replication and potentiates the anti-HCV activity of IFN-α through activation of the Jak/STAT signaling pathway independent of autophagy and apoptosis

    Chen, Y, et al. Virus Research 227(2017):231-239

  7. Identification and functional characterization of a novel thyrotropin receptor mutation (V87L) in a Chinese woman with subclinical hypothyroidism

    Zhang, HM, et al. Experimental and Therapeutic Medicine 131 (2017): 290-294

  8. A Novel Mechanism of Action for Salidroside to Alleviate Diabetic Albuminuria: Effects on Albumin Transcytosis across Glomerular Endothelial Cells

    Wu, D, et al. American Journal of Physiology-Endocrinology and Metabolism, 2016, 310(3): E225-E237.

  9. Expression of Smad1 in renal tubular epithelial cells in high glucose condition

    Chen Meilian, et al. China Tropical Medicine, 15.1 (2015): 8-10.

  10. Inflammasome-independent role of NLRP12 in suppressing colonic inflammation regulated by Blimp-1.

    Shi F, Yang Y, Kouadir M, et al. Oncotarget, 2016.

  11. MicroRNA-150 regulates blood–brain barrier permeability via Tie-2 after permanent middle cerebral artery occlusion in rats.

    Fang Z, He Q W, Li Q, et al. The FASEB Journal, 2016, 30(6): 2097-2107.

Cat #: A01010

  • Size
  • Price
  • 50μl $90
  • 200μl $260
  • 1ml $860
  • 10ml $4000

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