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Annexin V-AbFluor™ 405 Apoptosis Detection Kit

Annexin V-AbFluor™ 405 Apoptosis Detection Kit

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Specification

Product name Annexin V-AbFluor™ 405 Apoptosis Detection Kit
Applications notes Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary blue fluorescent dye AbFluor™ 405, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ 405 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.
Alternative Annexin 5; Annexin V; AbFluor™ 405

Product Properties

Kit components • Annexin V Binding Buffer (5x)
• Annexin V- AbFluor™ 405
• Propidium Iodide (PI)
Features & Benefits • Abbkine AbFluor™ 405 dye provides the same or better fluorescence and photostability as Alexa Fluor® 405, Cascade Blue® dye, DyLight™ 405
• Annexin V- AbFluor™ 405: Abs/Em = 404/431 nm; PI: Abs/Em = 535/617 nm (with DNA)
• Suitable for flow cytometry or fluorescence microscopy
Storage instructions Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.
Alternative Annexin 5; Annexin V; AbFluor™ 405

Image & description

Fig. Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit allows the identification and quantitation of intact cells, early apoptotic and late apoptotic or necrotic cells by flow cytometry or fluorescence microscopy.

Fig. Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit allows the identification and quantitation of intact cells, early apoptotic and late apoptotic or necrotic cells by flow cytometry or fluorescence microscopy.

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