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AMPKα1 Polyclonal Antibody

AMPKα1 Polyclonal Antibody

Views(256) Publications(0) Catalog no(ABP57190)
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Specification

Product name AMPKα1 Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Human, Mouse, Rat
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1) belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID 5562
Others The antibody detects endogenous AMPKα1 protein.
Accession Q13131

Image & description

Fig. Western blot analysis of 1) Hela, 2) 293T, 3) C2C12,  4) 3T3, 5) Rat Heart, 6) Rat Brain using AMPKα1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western blot analysis of 1) Hela, 2) 293T, 3) C2C12, 4) 3T3, 5) Rat Heart, 6) Rat Brain using AMPKα1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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