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Amphiphysin II Polyclonal Antibody

Amphiphysin II Polyclonal Antibody

Views(14) Publications(0) Catalog no(ABP53083)
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Specification

Product name Amphiphysin II Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Amphiphysin II
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative BIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.
Gene ID 274
Alternative BIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1
Others Amphiphysin II Polyclonal Antibody detects endogenous levels of Amphiphysin II protein.
Accession O00499

Image & description

Fig.1. Western Blot analysis of 4T1 cells using Amphiphysin II Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of 4T1 cells using Amphiphysin II Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

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