Login Register
English
0

Cart

$ 0

AKT Mouse Monoclonal Antibody (1H2)

AKT Mouse Monoclonal Antibody (1H2)

Views(74) Publications(0) Catalog no(ABM40276)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name AKT Mouse Monoclonal Antibody (1H2)
Immunogen Synthetic Peptide of AKT at AA range of 400-480
Host Mouse
Reactivity Human
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative AKT1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The serine-threonine protein kinase encoded by the AKT1 (AKT serine/threonine kinase 1) gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1.
Gene ID 207
Alternative AKT1
Others AKT protein detects endogenous levels of AKT1.
Accession P31749

Image & description

Fig.1. Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Fig.1. Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Fig.3. Western blot analysis of PC3 Cell Lysate using Akt Mouse mAb diluted at 1:1000.

Fig.3. Western blot analysis of PC3 Cell Lysate using Akt Mouse mAb diluted at 1:1000.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “AKT Mouse Monoclonal Antibody (1H2)”