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Actinin-α1/2/3/4 Polyclonal Antibody

Actinin-α1/2/3/4 Polyclonal Antibody

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Specification

Product name Actinin-α1/2/3/4 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human Actinin-α1/2/3/4 at AA range: 40-120
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; A

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 75 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.
Gene ID 87/88/89/81
Alternative ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; A
Others Actinin-α1/2/3/4 Polyclonal Antibody detects endogenous levels of Actinin-α1/2/3/4 protein.
Accession P12814/P35609/Q08043/O43707

Image & description

Fig.1. Western Blot analysis of various cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of A549 cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of A549 cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1:1000.

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