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ACCα Polyclonal Antibody

ACCα Polyclonal Antibody

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Specification

Product name ACCα Polyclonal Antibody
Immunogen Synthesized peptide derived from human ACCα around the non-phosphorylation site of S80
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for ACACA.
Gene ID 31
Alternative ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha
Others ACCα Polyclonal Antibody detects endogenous levels of ACCα protein.
Accession Q13085

Image & description

Fig.1. Western Blot analysis of various cells using ACCα Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using ACCα Polyclonal Antibody diluted at 1:1000.

Fig.2. Western blot analysis of 293T lysis using ACCα antibody. Antibody was diluted at 1:1000.

Fig.2. Western blot analysis of 293T lysis using ACCα antibody. Antibody was diluted at 1:1000.

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