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α-SMA Polyclonal Antibody

α-SMA Polyclonal Antibody

Views(923) Publications(0) Catalog no(ABP52852)
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Specification

Product name α-SMA Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human α-SMA
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin; ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA2; ACTSA; ACTVS; GIG46;

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. Actin alpha cardiac muscle 1/Actin alpha skeletal muscle/Actin aortic smooth muscle/Actin gamma-enteric smooth muscle encoded by ACTC1 belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
Gene ID 70
Alternative ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin; ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA2; ACTSA; ACTVS; GIG46;
Others α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.
Accession P68032
Observed Band(KD) 42

Image & description

Fig.1. Western Blot analysis of MFC cells using α-SMA Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of MFC cells using α-SMA Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

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