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α-SMA Polyclonal Antibody

α-SMA Polyclonal Antibody

Views(41) Publications(0) Catalog no(ABP52851)
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Specification

Product name α-SMA Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human α-SMA
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA2; ACTSA; ACTVS; GIG46; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actinACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA2; ACTSA; ACTVS; GIG46; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Actin aortic smooth muscle encoded by ACTA2 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Gene ID 59
Alternative ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA2; ACTSA; ACTVS; GIG46; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actinACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1; ACTA2; ACTSA; ACTVS; GIG46; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin
Others α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.
Accession P68133
Observed Band(KD) 42

Image & description

Fig.1. Western Blot analysis of MFC cells using α-SMA Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of MFC cells using α-SMA Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

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