|Product name||α-SMA Monoclonal Antibody|
|Reactivity||Human, Mouse, Rat|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:50000), IHC-P (1:1000-1:2000).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen|
|Storage buffer||PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The protein encoded by ACTA2 (actin, alpha 2, smooth muscle, aorta) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.|
|Others||The antibody detects endogenous α-SMA protein.|
Fig.1. Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain using α-SMA Monoclonal Antibody.
Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Cecal Tissue using α-SMA Monoclonal Antibody.
Fig.3. The picture was kindly provided by our customer.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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