Protein A/G is a genetically-engineered protein which combines the IgG binding domains of both Protein A and Protein G. The fusion protein is expressed in E. coli. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more general and convenient tool for investigating and purifying immunoglobulins. The surface is chemically modified with a proprietary method to minimize nonspecific binding of proteins. Also, Protein A/G binding to immunoglobulins is not as pH-dependant as Protein A.
PurKine™ Protein A/G Resin 4FF is effective for affinity purification of IgG from serum, ascites fluid, cell culture supernantant and other antibody samples. It can also apply to immunoprecipitation (IP) and co-immunoprecipitation (Co-IP). The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein A/G has been coupled. Each milliliter of drained resin will bind approximately 5-10 mg Human IgG. This product shows long term stability within pH range of 3 to 10.
PurKine™ Protein A/G Resin 4FF binds to all human IgG subclasses and binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM or serum albumin, making it ideal for purification of polyclonal or monoclonal IgG. In addition, the resin is very robust, which can tolerate up to 300cm/hour's flow rates. No matter scientific research or industrial production, this resin will meet requirements.