Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of immunoglobulins through interactions with the heavy chain. For antibody affinity purification, recombinant protein A is a better choice. Recombinant protein A is produced in E. coli that contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding.
Abbkine PurKine™ Protein A Resin has been immobilized for use in IgG purification applications. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein A has been coupled. Per mL of resin can capture more than 40mg human lgG. Tests confirm that performance equals or exceeds popular recombinant protein A resins from other suppliers. Column (PurKine™ Protein A Packed Column) which is prepacked with recombinant protein A resin is also supplied.
PurKine™ Protein A Purification system is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A is generally preferred for affinity purification of rabbit, Guinea pig, dog and cat IgG. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses.