IFKine™ Orange conjugated antibodies absorb light maximally at 555 nm and fluoresce with a peak around 570 nm. They are brighter than TRITC, Cy3. IFKine™ Orange conjugates are recommended for for all immunofluorescence procedures requiring a orange -fluorescing dye.
IFKine™ Orange Donkey Anti-Mouse IgG antibody reacts with whole molecule mouse IgG. It also reacts with light chains of all other mouse immunoglobulins. It's cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins. Abbkine suggested the starting dilutions for most applications are 1:50-1:1,000 .
Abbkine’s IFKine® is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine® secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.