IFKine™ Green conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 518 nm. They are brighter than FITC, Cy2, and DyLight 488. IFKine™ Green conjugates are recommended for for all immunofluorescence procedures requiring a green-fluorescing dye.
IFKine™ Green Donkey Anti-Goat IgG antibody reacts with whole molecule goat IgG. It also reacts with light chains of all other goat immunoglobulins. It's cross-reaction with human, mouse, rat, rabbit and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins. Optimal working dilutions should be determined experimentally by the investigator. Abbkine suggested the starting dilution is 1:50-1:1,000 for most applications.
Abbkine’s IFKine® is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine® secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.